中国病理生理杂志2023，Vol.39Issue(12)：2158-2164,7.DOI:10.3969/j.issn.1000-4718.2023.12.006
紫云英苷诱导炎性痛小鼠前扣带回皮质星形胶质细胞自噬
Astragalin induces autophagy of astrocytes in anterior cingulate cortex of inflammatory pain mice
摘要
Abstract
AIM:To investigate the effects of astragalin(AST)on activation status of astrocytes and the ex-pression level of autophagy-related proteins in the cortex of the anterior cingulate cortex of mice with a complete Freund's adjuvant(CFA)-induced inflammatory pain model.METHODS:Twenty-four 6-month-old male C57BL/6 mice were ran-domly divided into four groups:control group,saline group,CFA model group and CFA+60 mg/kg AST administration group,and six mice in each group.Mice in the AST administration group received 60 mg/kg AST by intraperitoneal injec-tion on a body weight basis for 21 d.The paw withdrawal threshold in each group of mice was evaluated by the von Frey test.The expression levels of autophagy-related factors LC3,p62,ATG12 and beclin-1,and astrocyte activation were de-tected by multiplex immunofluorescence staining in the anterior cingulate cortex of mice in each group.Western blot was used to measure the levels of autophagy-related proteins LC3,p62,ATG12 and beclin-1 in the anterior cingulate cortex of mice in each group.RESULTS:Behavioural tests showed that AST significantly increased mechanical pain thresholds in CFA mice(P＜0.05).The results from multiple immunofluorescent staining showed that AST significantly increased the fluorescence intensity of LC3(P＜0.01),ATG12(P＜0.01)and beclin-1(P＜0.05),attenuated the fluorescence intensi-ty of p62(P＜0.05),and inhibited the activation of astrocytes in the anterior cingulate cortex of CFA mice.Western blot results further confirmed that AST significantly increased the expressions of LC3(P＜0.01),ATG12(P＜0.01),beclin-1(P＜0.01),and decreased the expression of p62(P＜0.05)in the anterior cingulate cortex of CFA mice.CONCLU-SION:AST relieves CFA-induced inflammatory pain of mice,and its analgesic mechanism may be related to the inhibi-tion of activation of cortical astrocytes in the anterior cingulate cortex and the promotion of autophagy in CFA mice.关键词
紫云英苷/炎症性疼痛/前扣带回皮质/星形胶质细胞/细胞自噬Key words
astragalin/inflammatory pain/anterior cingulate cortex/astrocytes/autophagy分类
医药卫生引用本文复制引用
林佳泓,王姝涵,张润恒,杨翠珠,杨雅琪,周畅,唐佩,刘靖,马宇昕..紫云英苷诱导炎性痛小鼠前扣带回皮质星形胶质细胞自噬[J].中国病理生理杂志,2023,39(12):2158-2164,7.基金项目
广东省医学科学技术研究基金(No.B2022076) （No.B2022076）
广东省中医药局中医药科研项目(No.20231201 （）
No.20221213) （）
广东省普通高校特色创新类项目(No.2023KTSCX057) （No.2023KTSCX057）
广州市基础与应用基础研究项目(No.202201010181 （）
No.202201010299) （）
