中国病理生理杂志2023，Vol.39Issue(12)：2234-2241,8.DOI:10.3969/j.issn.1000-4718.2023.12.014
基于AMPK信号通路探究樱桃李多酚提取物对胰岛素抵抗HepG2细胞糖代谢的作用
Effects of Prunus cerasifera Ehrh.polyphenol extract on glucose metabo-lism in insulin-resistant HepG2 cells based on AMPK signaling pathway
摘要
Abstract
AIM:To investigate the effects of Prunus cerasifera Ehrh.polyphenol extract(PCE)on glucose metabolism in insulin-resistant HepG2(IR-HepG2)cells.METHODS:An IR-HepG2 cells model was established using high sugar and high insulin induction;a control group,an insulin resistance(IR)model group,a PCE treatment group with different concentrations,and a metformin treatment group were set up.Cells were treated with 50,100,200,and 400 mg/L of polyphenol extract for 24 hours,respectively,to detect their effects on cellular glucose consumption.At the same time,use thiazole blue(MTT)colorimetry to evaluate cell viability.Real-time PCR and Western blot were used to detect expression of glucose metabolism-related mRNA and proteins.RESULTS:Prunus cerasifera Ehrh.polyphenol ex-tract can significantly increase glucose consumption in IR-HepG2 cells,and cell activity tends to increase and then decline as its concentration increases;At mRNA and protein levels,in addition to significantly increasing the expression of phos-phorylated adenosine monophosphate activated protein kinase(p-AMPK)protein in IR-HepG2 cells,it also decrease gly-cogen synthase kinase 3β(GSK3β)in glycogen synthesis and gluconeogenesis pathways,forked protein O1(FoxO1),peroxisome proliferator activates gamma co-receptor activation 1-alpha(PGC-1α),glucose-6-phosphatase(G6Pase)and phosphoenol pyruvate carboxykinase(PEPCK)expression.CONCLUSION:Prunus cerasifera Ehrh.polyphenol extract can activate the AMPK signaling pathway in IR-HepG2 cells,to further inhibit gluconeogenesis and glycogen synthesis pathways,thereby improving the insulin resistance state of HepG2 cells.关键词
樱桃李多酚提取物/HepG2细胞/胰岛素抵抗/糖代谢/AMP活化蛋白激酶Key words
Prunus cerasifera Ehrh.polyphenol extracts/HepG2 cells/insulin resistance/glucose metabo-lism/AMP-activated protein kinase分类
医药卫生引用本文复制引用
苏黑艳·帕尔哈提,张姣姣,王俊人,赵家琪,李艳红..基于AMPK信号通路探究樱桃李多酚提取物对胰岛素抵抗HepG2细胞糖代谢的作用[J].中国病理生理杂志,2023,39(12):2234-2241,8.基金项目
新疆维吾尔自治区自然科学基金面上项目(No.2022D01A100) （No.2022D01A100）
新疆维吾尔自治区高校科研计划项目(No.XJEDU2023J031) （No.XJEDU2023J031）
新疆师范大学重点实验室招标课题(No.XJTSWZ-2021-01) （No.XJTSWZ-2021-01）
新疆师范大学2022年硕士研究生科研创新项目(No.XSY202201008) （No.XSY202201008）
