中国动物检疫2023，Vol.40Issue(12)：22-27,6.DOI:10.3969/j.issn.1005-944X.2023.12.005
新疆阿拉尔市流浪猫源蜱种类鉴定及其携带立克次体PCR检测
Identification of Tick Species of Stray Cat Origin and PCR Detection for Rickettsia Carried by Them in Alar City,Xinjiang Uygur Autonomous Region
摘要
Abstract
In order to investigate tick species on the body surface of cats and Rickettsia carried by them,tick samples were collected from stray cats in a residential area of Alar,Xinjiang to identify tick species using the morphological and molecular biology method,and to detect the Rickettsia carried by ticks using PCR.Then the genotype of Rickettsia was identified through sequence matching,and a genetic evolution tree was constructed to analyze its molecular genetic characteristics.The results revealed that one of the 4 stray cats had 3 ticks parasitized on its body surface,which were preliminarily identified as Rhipicephalus turanicus through morphological observation;based on the mitochondrial SSU rRNA and COX Ⅰ genes,the DNA samples of the three ticks were amplified by PCR,and the ticks were finally identified as Rhipicephalus turanicus.Based on OmpA and OmpB genes of Rickettsia,the DNA samples of the three ticks were amplified by PCR and their sequences were compared and analyzed,it was found that the Rickettsia sequence detected in the DNA of two ticks was 100%homologous to the Candidatus R.barbariae from Rhipicephalus turanicus in China and Rhipicephalus annulatus in Lebanon,hence the Rickettsia was identified as Candidatus R.barbariae.In conclusion,there were Rhipicephalus turanicus parasitized on the body surface of stray cats in Alar,which could carry Candidatus R.barbariae.A basic data was thus provided for researches on parasitic ticks of cat origin and their pathogen carrying status in China.关键词
图兰扇头蜱/斑点热群立克次体/流浪猫/暂定巴布瑞立克次体Key words
Rhipicephalus turanicus/spotted fever group Rickettsia/stray cat/Candidatus R.barbariae分类
农业科技引用本文复制引用
司俊飞,赫永强,张振杰,朱婷婷,张旭,曹梦雅,赵爱云,齐萌..新疆阿拉尔市流浪猫源蜱种类鉴定及其携带立克次体PCR检测[J].中国动物检疫,2023,40(12):22-27,6.基金项目
新疆生产建设兵团第九师科技计划项目(2023JS002) （2023JS002）
