中国动物检疫2023，Vol.40Issue(12)：80-85,6.DOI:10.3969/j.issn.1005-944X.2023.12.016
禽腺病毒4型检测方法研究进展
Progress in Researches on FAdV-4 Detection Methods
摘要
Abstract
Avian adenovirus type 4(FAdV-4)is a common immunosuppressive virus in poultry,also one of the major viral pathogens posing a threat to the healthy development of poultry industry.FAdV-4 mainly causes hydropericardium-hepatopathycomplex syndrome(HHS),with main clinical manifestations including hydropericardium and hepatitis,and a mortality rate of up to 80%,seriously endangering the healthy development of poultry industry.FAdV-4 could be effectively monitored by rapid and accurate detection methods,which lay the foundation for disease diagnosis,prevention and control as well as further researches.In the paper,global FAdV-4 detection techniques were summarized,including neutralization test,agar gel immunodiffusion test,enzyme-linked immunosorbent assay,indirect immunofluorescence assay and other serological detection techniques,as well as polymerase chain reaction(PCR),real-time fluorescent quantitative PCR,loop-mediated isothermal amplification and other molecular biological diagnostic techniques,hoping to offer a reference for the researches on rapid detection techniques of FAdV-4.关键词
禽腺病毒4型/检测方法/血清学/分子生物学Key words
FAdV-4/detection method/serology/molecular biology分类
农业科技引用本文复制引用
孟鸽,刘冠慧,李阳,张同锋,尚佳静,张永英,罗娟,封莹洁,于晓慧,蒋文明,刘华雷..禽腺病毒4型检测方法研究进展[J].中国动物检疫,2023,40(12):80-85,6.基金项目
山东省重点研发计划项目(2022CXGC010606) （2022CXGC010606）
