中国动物检疫2023，Vol.40Issue(12)：98-103,6.DOI:10.3969/j.issn.1005-944X.2023.12.019
胸膜肺炎放线杆菌感染猪肺巨噬细胞相关细胞因子表达水平初探
Preliminary Study on the Expression Level of Cytokines Produced by Porcine Pulmonary Macrophages Infected with Actinobacillus pleuropneumoniae
刘红洋 1姜艳雪 2邵清 1邴新帅 3王秋东 4巩俊明 1朱奕霏 5王羽辉 1田喜荣 1金哲1
作者信息
- 1. 双辽市动物疫病预防控制中心,吉林双辽 136400
- 2. 双辽市动物检疫站,吉林双辽 136400
- 3. 双辽市畜禽屠宰工作服务中心,吉林双辽 136400
- 4. 双辽市畜牧工作总站,吉林双辽 136400
- 5. 吉林省动物疫病预防控制中心,吉林长春 130000
- 折叠
摘要
Abstract
In order to further investigate the pathogenic mechanism of Actinobacillus pleuropneumoniae(APP)infection in piglets,the changes in the expression levels of IL-1β,IL-6,IL-8 and caspase-3 genes in infected piglets were investigated by SYBR green fluorescence quantitative PCR assay.Porcine alveolar macrophages(PAMs)were obtained through bronchoalveolar lavage fluid and infected by APP at a dose of MOI = 10,then the expression levels of relevant cytokine genes(IL-1β,IL-6,IL-8 and caspase-3)were detected.The results showed that the amounts of gene expression of IL-1β,IL-6,IL-8 and caspase-3 were increasing at 3～12 h after APP infection,and reached a peak at 12～24 h.In conclusion,IL-1β,IL-6 and IL-8 played an important role in clearing pathogenic bacteria by hosts at early stage of APP infection.A foundation was laid for further exploration of APP's pathogenic mechanism.关键词
胸膜肺炎放线杆菌/IL-1β/IL-6/IL-8/caspase-3/SYBR Green荧光定量PCRKey words
Actinobacillus pleuropneumoniae/IL-1β/IL-6/IL-8/caspase-3/SYBR green fluorescence quantitative PCR分类
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刘红洋,姜艳雪,邵清,邴新帅,王秋东,巩俊明,朱奕霏,王羽辉,田喜荣,金哲..胸膜肺炎放线杆菌感染猪肺巨噬细胞相关细胞因子表达水平初探[J].中国动物检疫,2023,40(12):98-103,6.
