中国感染控制杂志2023，Vol.22Issue(12)：1475-1482,8.DOI:10.12138/j.issn.1671-9638.20234213
匹莫齐特对金黄色葡萄球菌的体外和体内抗菌研究
In vitro and in vivo antimicrobial activity of pimozide against Staphylo-coccus aureus
摘要
Abstract
Objective To explore the in vitro and in vivo antimicrobial activity of antipsychotic agent pimozide against Staphylococcus aureus(S.aureu).Methods The minimal inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)of pimozide were determined by micro-dilution assay.Biofilm was cultured in 96-well cell culture plate,and the anti-biofilm activity of pimozide was detected by turbidimetry.The effect of pimozide on biofilm was further observed through laser confocal microscopy and SYTO9/PI staining.Combined antimicrobial effect of pimozide and other antimicrobial agents was detected by chessboard dilution method,and cytotoxicity of pimozide was detected by CCK-8 assay kit.A model of skin abscess was constructed,in vivo antimicrobial activity and toxicity of pimozide was tested.Results Pimozide showed significant dose-dependent antimicrobial activity against S.aureu,with a MIC of 8-16 μg/mL.It could significantly inhibit the formation of S.aureu biofilm and disperse the formed biofilm.The combination of pimozide and doxycycline has a synergistic antimicrobial effect in vitro,with a synergistic antimicrobial index of 0.5.It can significantly reduce the bacterial load in mouse abscess tissue in vivo,and reduce the live bacterial count from(8.25±0.13)lgarithmic value of CFU/abscess to(3.31± 0.81)logarithmic value of CFU/abscess(q=3.74,P＜0.05).The cytotoxicity of pimozide was extremely low,with a half inhibitory concentration of 64 μg/mL on cells.Conclusion Pimozide exhibits significant antimicrobial activity in vitro and in vivo with extremely low toxicity,thus is promising for the treatment of S.aureu-related local infection in psychiatric patients.关键词
匹莫齐特/金黄色葡萄球菌/抗菌作用/生物膜/联合药敏试验Key words
pimozide/Staphylococcus aureus/antimicrobial action/biofilm/combined antimicrobial susceptibility testing分类
医药卫生引用本文复制引用
陈体,段瑶,张晓洁,佘鹏飞,李颖佳..匹莫齐特对金黄色葡萄球菌的体外和体内抗菌研究[J].中国感染控制杂志,2023,22(12):1475-1482,8.基金项目
湖南省自然科学基金项目(2021JJ40944) （2021JJ40944）
