中国骨伤2023，Vol.36Issue(12)：1136-1141,6.DOI:10.12200/j.issn.1003-0034.2023.12.006
肩袖损伤患者肩袖组织促氧化状态及Beclin-1和mTOR的表达
Pro-oxidation status of rotator cuff tissue and expression of Beclin-1 and mTOR in patients with rotator cuff injury
摘要
Abstract
Objective To explore pro-oxidative state of rotator cuff tissue and expression levels of Beclin-1 and mam-malian target of rapamycin(mTOR)in patients with acute and chronic rotator cuff injury,and then analyzed relationship be-tween rotator cuff injury and oxidative stress and autophagy.Methods Forty patients with rotator cuff injury were seleceted from July 2019 to December 2020,and divided into male chronic injury group,male acute injury group,female chronic injury group,and female acute injury group,10 patients in each group.All patients were performed rotator cuff repair under arthroscopy.The sample of tendon at the rotator cuff injury site of the patient was taken during operation,and total reactive oxygen species(ROS)and superoxide dismutase(SOD)were detected by detection kit;expression of Beclin-1 and mTOR mRNA were detected by reverse transcription-polymerase chain reaction(RT-PCR),and Westem-blot was applied to detect protein expression of Beclin-1 and p-mTOR/mTOR.Results There were no significant difference in expression of ROS,SOD,Beclin-1mRNA and mTOR mRNA between male and female chronic injury groups,and between male and female acute injury groups(P＞0.05);ROS,SOD and Beclin-1mRNA in male chronic injury group were higher than those in male chronic injury group,while mTOR mRNAand protein decreased(P＜0.05);ROS,SOD and Beclin-1 mRNA in female chronic injury group were up-regulated compared with female acute injury group,while mTOR mRNA was down-regulated(P＜0.05).Conclusion Chronic rotator cuff injury is more likely to stimulate the pro-oxidation state of rotator cuff tissue than acute rotator cuff injury,which could up-regulating expression of autophagy factor Beclin-1 and down-regulating expression of mTOR.Therefore,pa-tients with chronic rotator cuff injury may have higher levels of oxidative stress and autophagy.关键词
肩袖损伤/Beclin-1蛋白/雷帕霉素靶蛋白/活性氧/超氧化物类Key words
Rotator cuff injury/Beclin-1/Target of rapamucin proteins/Reactive oxygen species/Superoxides分类
医药卫生引用本文复制引用
刘治军,魏合伟,郑维蓬,万雷,廖志浩,陈胜..肩袖损伤患者肩袖组织促氧化状态及Beclin-1和mTOR的表达[J].中国骨伤,2023,36(12):1136-1141,6.基金项目
广东省中药管局科研项目(编号:20203011) （编号:20203011）
广东省医学科学技术研究基金项目(编号:B2020030)Scientific Research Project of Guangdong Administration of Traditional Chinese Medicine(No.20203011) （编号:B2020030）
