中国临床药理学与治疗学2023，Vol.28Issue(12)：1321-1330,10.DOI:10.12092/j.issn.1009-2501.2023.12.001
基于网络药理学探讨平消胶囊通过AKT1/β-catenin信号促进乳腺癌凋亡的作用机制
Mechanism of Pingxiao capsule promoting breast cancer apoptosis through AKT1/β-catenin signal based on network pharmacology
摘要
Abstract
AIM:To explore the potential mecha-nism of action of Pingxiao capsule in the treatment of breast cancer.METHODS:TCMSP,TCM-ID,Gene-Cards and other databases were used to screen the related targets of Pingxiao capsule and breast can-cer.Cytoscape software builds drug-target-disease networks.R software was used for GO and KEGG analysis.Autodock Vina and Pymol software were used for molecular docking and visualization of Pingxiao capsule active ingredients and core tar-gets.Core targets were analyzed by R software sur-vival package,and genes closely related to overall survival time were screened out.Cell viability was detected by CCK-8 method.Flow cytometry was used to detect cell apoptosis.The protein expres-sion levels of p-AKT1,β-catenin and cyclinD1 were detected by Western blot.RESULTS:A total of 194 drug targets were screened,1612 disease targets were identified,127 intersection targets were iden-tified by Venn diagram,and 20 core targets were TB53,AKT1,TNF,CASP3,etc.GO analysis was main-ly related to oxidative stress response,cell regula-tion of chemical reaction and other biological activi-ties.KEGG analysis was mainly related to PI3K-AKT signaling pathway,TNF signaling pathway,IL-17 sig-naling pathway and other pathways.Molecular docking results showed that the active constituents were well combined with the core targets AKT1,MAPK1 and RELA.Cell experiments showed that quercetin(40,80,120 μmol/L)promoted apoptosis of breast cancer cells.Western blot analysis showed that the protein expressions of p-AKT1,β-catenin and cyclinD1 decreased with different con-centrations of quercetin treated for 48h.CONCLU-SION:Network pharmacology and cell experiments confirmed that Pingxiao capsule may exert its anti-breast cancer effect by regulating AKT1/β-catenin signaling pathway.关键词
平消胶囊/乳腺癌/网络药理学/分子对接/AKT1/β-cateninKey words
Pingxiao capsule/breast cancer/net-work pharmacology/molecular docking/AKT1/β-catenin分类
医药卫生引用本文复制引用
陈洪晓,刘苏,金乐,张慧慧,张蕾,陈昭琳..基于网络药理学探讨平消胶囊通过AKT1/β-catenin信号促进乳腺癌凋亡的作用机制[J].中国临床药理学与治疗学,2023,28(12):1321-1330,10.基金项目
安徽省自然科学基金项目(2208085MH252) （2208085MH252）
安徽省重点研究与开发计划项目(201904a07020092) （201904a07020092）
安徽省自然科学基金项目(2108085MH311) （2108085MH311）
