中国临床药理学与治疗学2023，Vol.28Issue(12)：1339-1346,8.DOI:10.12092/j.issn.1009-2501.2023.12.003
常春藤皂苷元通过调控巨噬细胞Mincle介导的炎症减轻银屑病小鼠皮肤损伤的作用机制
Hederagenin improves psoriasis skin lesions by inhibiting macro-phage mincle-mediated inflammation
摘要
Abstract
AIM:To observe the effects and mech-anisms of hederagenin(HDG)in improving psoria-sis skin lesions and inflammation in mice.METH-ODS:A mouse model of psoriasis was established by depilation of the back and continuous applica-tion of imiquimod for 7 days in C57 mice.After modeling,HDG was administered orally(low dose:25 mg·kg-1·d-1 and high dose:50 mg·kg-1·d-1)1 hour later,and a positive control group was treated with dexamethasone.After 7 days of drug interven-tion,pathological,immunohistochemical,immuno-fluorescence,ELISA,real-time quantitative PCR,and Western blot analyses were performed on the skin lesions of each group of mice.RESULTS:Com-pared with the model group,the HDG intervention group showed varying degrees of improvement in skin pathological damage and inflammatory cell in-filtration.Real-time PCR and ELISA results of skin tissue suspension confirmed that the mRNA and protein levels of inflammatory factors IL-1β,IL-6,and TNF-α in mouse skin were reduced in the HDG intervention group compared to the model group,indicating a significant anti-inflammatory effect of HDG.Immunohistochemical and Western blot re-sults showed that compared with the normal group,the protein expression of Mincle in the skin of the model group mice was significantly in-creased,which was significantly down-regulated af-ter HDG intervention.Immunofluorescence con-firmed the co-localization of Mincle expression and macrophage marker F4/80 in the skin of the model group.Western blot analysis revealed that HDG not only down-regulated the protein level of Mincle in the treatment group but also reduced the protein phosphorylation levels of downstream signaling molecules Syk and NF-κB.CONCLUSION:Heder-agenin intervention can significantly inhibit patho-logical damage and macrophage-related inflamma-tion in psoriasis,and its potential molecular mecha-nism may be related to the down-regulation of the Mincle/Syk/NF-κB signaling pathway.关键词
常春藤皂苷元/Mincle/皮肤损伤/炎症/银屑病Key words
hederagenin/mincle/macrophage/inflammation/psoriasis分类
医药卫生引用本文复制引用
何馨雨,钟霞,刘鹏,谭睿陟..常春藤皂苷元通过调控巨噬细胞Mincle介导的炎症减轻银屑病小鼠皮肤损伤的作用机制[J].中国临床药理学与治疗学,2023,28(12):1339-1346,8.基金项目
四川省自然科学基金资助项目(2022NSFSC0606) （2022NSFSC0606）
