中国免疫学杂志2023，Vol.39Issue(12)：2477-2482,6.DOI:10.3969/j.issn.1000-484X.2023.12.003
β-谷甾醇对OVA诱导的过敏性哮喘大鼠模型中Th17/Treg免疫失衡的调节
Regulation of Th17/Treg immune imbalance by β-sitosterol in an OVA-induced allergic asthma rat model
摘要
Abstract
Objective:To explore the interventional effect of β-sitosterol on ovalbumin(OVA)-induced allergic asthma rats and its potential mechanism.Methods:SD male rats were randomly divided into normal group(CON),model group(M),positive drug dexamethasone group(DEX,0.075 mg/kg)and β-sitosterol group(Sit,50 mg/kg).A rat model of allergic asthma was estab-lished by intraperitoneal injection of OVA with aluminum hydrogen solution,and nebulized inhalation of OVA to stimulate.Rats were given intragastric administration 30 min before aerosol challenge,and after continuous administration for 7 days,the indicators of cough and asthma and tracheal phenol red excretion were detected.HE staining was used to observe pathological changes of lung tis-sue.Flow cytometry was used to detect reactive oxygen species(ROS)generation,apoptosis level and ratios of Th17 and Treg cells in peripheral blood.Biochemical method was used to detect contents of MDA,and activities of T-SOD and GSH-Px in rat lung tissues.ELISA was used to detect levels of Th17 and Treg-related cytokines(TNF-α,IL-4,IL-6,IL-17A,and IL-35).Results:Compared with model group,β-sitosterol significantly prolonged the incubation period of cough and gasp in rats with allergic asthma,reduced the frequency of cough and gasping,and promoted the excretion of phenol red in trachea;significantly reduced inflammatory infiltration in lung tissue of asthmatic rats;observably reduced MDA content in lung tissue,ROS of primary lung cell and apoptosis levels of asthmatic rats,increased the activities of T-SOD and GSH-Px;markedly reduced proportion of Th17 cells and levels of pro-inflammatory cyto-kines TNF-α,IL-4,IL-6 and IL-17A,increased proportion of Treg cells and levels of anti-inflammatory cytokine IL-35.Conclusion:β-sitosterol can ameliorate airway inflammation and oxidative damage in OVA-induced allergic asthmatic rats,and its mecha-nism may be related to the regulation of β-sitosterol on Th17/Treg immune imbalance and oxidative stress response.关键词
β-谷甾醇/过敏性哮喘/OVA/Th17/Treg免疫失衡Key words
β-sitosterol/Allergic asthma/OVA/Th17/Treg immune imbalance分类
医药卫生引用本文复制引用
贾菊芳,冯卫生,曾梦楠,张贝贝,王茹,刘萌,郭彭莉,张钦钦,张风宇,郑晓珂..β-谷甾醇对OVA诱导的过敏性哮喘大鼠模型中Th17/Treg免疫失衡的调节[J].中国免疫学杂志,2023,39(12):2477-2482,6.基金项目
本文为国家重点研发计划项目:中医药现代化研究重点专项(2019YFC1708802) （2019YFC1708802）
河南省重大科技专项项目(171100310500) （171100310500）
河南省高层次人才特殊支持计划"中原千人计划"-中原领军人才项目(ZYQR201810080). （ZYQR201810080）
