中国免疫学杂志2023，Vol.39Issue(12)：2624-2626,2630,4.DOI:10.3969/j.issn.1000-484X.2023.12.028
嵌套EP管法更快速获取更多小鼠骨髓细胞
Tube-nesting EP:A new faster method to get more mouse bone marrow cells
摘要
Abstract
Objective:To explore a method to obtain more mouse bone marrow cells more quickly.Methods:Take both lower limbs of the same mouse,the bone marrow cells were obtained by either the tube-nesting EP method or the traditional syringe irrigation method,recorded the time and quantity of bone marrow cells,to analyze the proportion of viable cells by flow cytometry,and to detect the proliferation of these bone marrow cells used CCK-8 method.Results:Compared with the traditional syringe irrigation method,the time of obtaining mouse bone marrow cells by tube-nesting EP method was shorter(P<0.000 1).Compared with the traditional syringe irrigation method,the number of bone marrow cells obtained by tube-nesting EP method was more(P<0.05).The proportion of live cells and proliferation ability of bone marrow cells obtained by the two methods were identical.Conclusion:Tube-nesting EP method is a new faster method to get more mouse bone marrow cells.关键词
嵌套EP管法/骨髓细胞/小鼠Key words
Tube-nesting EP/Bone marrow cells/Mouse分类
生物科学引用本文复制引用
徐启英,王卓亚,冶怡,乌仁塔娜..嵌套EP管法更快速获取更多小鼠骨髓细胞[J].中国免疫学杂志,2023,39(12):2624-2626,2630,4.基金项目
本文为青海省科协中青年科技人才托举工程项目(2022QHS-KXRCTJ44) （2022QHS-KXRCTJ44）
青海省高等学校省级骨干教师培养对象项目. （）
