农学学报2023，Vol.13Issue(12)：20-27,8.
抗根肿病大白菜材料SSR分子标记的初步筛选
Preliminary Screening of SSR Molecular Markers for Resistance to Clubroot in Brassica rapa L.ssp.pekinensis
摘要
Abstract
In order to identify the clubroot resistance gene in Chinese cabbage cultivar CCR12049 and develop molecular markers,the following materials were employed:CCR12049,a high-generation inbred line with high resistance to clubroot disease,CM12081,a high-generation inbred line with high susceptibility to clubroot disease,F1 generation resulting from the cross between CCR12049 and CM12081,and the F2 generation segregating populations created through selfing of the F1 generation.Through artificial inoculation identification,polyacrylamide gel electrophoresis,and sequence alignment,it was demonstrated that the disease resistance in tested materials was governed by a dominant single gene.36 primer pairs were initially tested on both the parental lines and F1 generation,4 primer pairs exhibiting polymorphism were identified.Subsequent validation in the F2 generation revealed that only one pair(cr-26)yielded amplification results consistent with the observed phenotype in the F2 generation.Through sequencing comparison of the PCR products obtained from the two parental lines and F1,it was observed that the sequences of the resistant parents and F1 were identical within the range of 95 bp to 111 bp.Conversely,in the susceptible materials,a deletion of 17 bases(TCTCTATCTCTTACGCA)was identified.These findings suggest that the resistance observed in the impermeable materials may be attributed to the insertion of these 17 bases.This marker has the capability to distinguish the resistant materials and can serve as an SSR marker for the initial screening of clubroot resistance in Chinese cabbage.关键词
大白菜/根肿病/SSR分子标记/遗传规律/序列比对Key words
Chinese cabbage/clubroot disease/simple sequence repeats(SSR)molecular marker/inheritance law/sequence alignment分类
农业科技引用本文复制引用
兰梅,张丽琴,徐学忠,胡靖锋,杨红丽,杨鼎,李崇娟,和江明..抗根肿病大白菜材料SSR分子标记的初步筛选[J].农学学报,2023,13(12):20-27,8.基金项目
财政部和农业农村部"国家现代农业产业技术体系"(CARS-23-G-33) （CARS-23-G-33）
云南省科技厅科技计划项目基础研究专项"青花菜花球遗传图谱的构建及硫代葡萄糖苷相关性状的QTL定位分析"(202301AT070089) （202301AT070089）
云南省发展和改革委员会"万人计划产业领军人才"(YNWR-CYJS-2018-040) （YNWR-CYJS-2018-040）
云南省科技厅科技计划项目"通海县蔬菜产业科技特派团"(202204BI090023) （202204BI090023）
云南省科技厅重大科技专项计划子课题"云南野、特蔬菜种质保护及研究利用"(202302AE090006-4). （202302AE090006-4）
