中国农业大学学报2024，Vol.29Issue(1)：53-65,13.DOI:10.11841/j.issn.1007-4333.2024.01.05
甜玉米轮作配施万寿菊秸秆返田防治白菜根肿病机理初探
Preliminary study on the mechanism of controlling Chinese cabbage clubroot disease by applying marigold straw in sweet corn rotation
摘要
Abstract
The aims of this study were to investigate the control mechanism of sweet corn rotation combined with marigold straw returning mode on cabbage clubroot disease,and provide theoretical basis and technical support for the planting mode.Fluorescence quantitative PCR technology,non-target metabolomics technology,high-throughput sequencing technology and field test verification were used in this study to explore the field control effect of sweet corn rotation combined with marigold straw returning treatment(CrMs)on Chinese cabbage clubroot,the number of resting spores and the differences in metabolites and microbial species in rhizosphere soil of Chinese cabbage.The results showed that:The field control effect of CrMs treatment on cabbage clubroot was 61.11％.The number of resting spores in the soil decreased from 1×10592spores per gram to 1×103.73spores per gram.CrMs treatment changed the metabolite components in the rhizosphere soil of Chinese cabbage,increased the contents of metabolites such as chenodeoxycholic acid,alpha-Linolenic acid and 4-lsopropylbenzoic acid,and decreased the contents of metabolites such as oleamide and melibiose.Alpha-Linolenic acid and chenodeoxycholic acid were negatively correlated with the relative abundance of Plasmodiophora,and oleamide was positively correlated with the relative abundance of Plasmodiophora.In addition,CrMs treatment enriched the rhizosphere microbial diversity of Chinese cabbage,improved the composition of the soil microbial community,significantly increased the relative abundance of Bacillus,Bradyrhizobium,Lysobacter and Streptomyces,and decreased the relative abundance of Plasmodiophora,Fusarium,and Helicotylenchus.In summary,the new mode of sweet corn rotation+marigold straw returning to the field is an important cultivation measure to control the Chinese cabbage clubroot,which provides a key technical reference for the green prevention and control of the clubroot.关键词
白菜根肿病/轮作/代谢组/微生物多样性Key words
Chinese cabbage clubroot/rotation/metabolomics/microbial diversity分类
农业科技引用本文复制引用
周星海,魏方俊,张晋豪,贺赛雅,邱玥,艾瑛,魏兰芳,姬广海..甜玉米轮作配施万寿菊秸秆返田防治白菜根肿病机理初探[J].中国农业大学学报,2024,29(1):53-65,13.基金项目
国家自然科学基金项目(32260701) （32260701）
国家重点研发计划项目(2019YFD1002000) （2019YFD1002000）
