甜玉米轮作配施万寿菊秸秆返田防治白菜根肿病机理初探OACSTPCD

The aims of this study were to investigate the control mechanism of sweet corn rotation combined with marigold straw returning mode on cabbage clubroot disease,and provide theoretical basis and technical support for the planting mode.Fluorescence quantitative PCR technology,non-target metabolomics technology,high-throughput sequencing technology and field test verification were used in this study to explore the field control effect of sweet corn rotation combined with marigold straw returning treatment(CrMs)on Chinese cabbage clubroot,the number of resting spores and the differences in metabolites and microbial species in rhizosphere soil of Chinese cabbage.The results showed that:The field control effect of CrMs treatment on cabbage clubroot was 61.11％.The number of resting spores in the soil decreased from 1×10592spores per gram to 1×103.73spores per gram.CrMs treatment changed the metabolite components in the rhizosphere soil of Chinese cabbage,increased the contents of metabolites such as chenodeoxycholic acid,alpha-Linolenic acid and 4-lsopropylbenzoic acid,and decreased the contents of metabolites such as oleamide and melibiose.Alpha-Linolenic acid and chenodeoxycholic acid were negatively correlated with the relative abundance of Plasmodiophora,and oleamide was positively correlated with the relative abundance of Plasmodiophora.In addition,CrMs treatment enriched the rhizosphere microbial diversity of Chinese cabbage,improved the composition of the soil microbial community,significantly increased the relative abundance of Bacillus,Bradyrhizobium,Lysobacter and Streptomyces,and decreased the relative abundance of Plasmodiophora,Fusarium,and Helicotylenchus.In summary,the new mode of sweet corn rotation+marigold straw returning to the field is an important cultivation measure to control the Chinese cabbage clubroot,which provides a key technical reference for the green prevention and control of the clubroot.