中国农业大学学报2024，Vol.29Issue(2)：122-130,9.DOI:10.11841/j.issn.1007-4333.2024.02.12
捻转血矛线虫耐药虫株与敏感虫株circRNAs差异表达分析
Differential expression of circRNAs in resistant and sensitive strains of Haemonchus contortus
摘要
Abstract
The study was to investigate the role of differentially expressed circRNAs in the regulation of drug resistance between ivermectin sensitive and resistant strains of Haemonchus contortus.In this study,sensitive and resistant strains of H.contortus were taken as research objects.Illumina Hiseq 2 000 platform was used for high-throughput sequencing and cDNA library construction,and relevant bioinformatics software was used to analyze the difference of circRNAs.Simultaneously,GO and KEGG pathway functions of the source genes were enriched.TargetScan and Miranda software were used to predict the miRNAs targeted by key circRNAs,and the role of circRNAs-miRNAs was explored.Subsequently,5 circRNAs with significant differences were randomly selected for RT-qPCR detection and consistency assessment with transcriptome sequencing result.The results showed that:1)A total of 677 differentially expressed circRNAs were screened between sensitive and resistant strains,of which 47 were significantly different,23 were significantly up-regulated,and 24 were significantly down-regulated.2)GO enrichment analysis of all differentially derived circRNAs showed that 336 biological processes,59 cell components and 110 molecular functions were enriched,which were mainly related to metabolic processes,cell membrane structure and catalytic activity.KEGG enrichment showed that 257 pathways were mainly enriched,most of which were related to metabolism(fatty acid biosynthesis,glycerophospholipid metabolism and propionate metabolism),glucagon signaling pathway and AMPK signaling pathway.3)circRNAs-miRNAs regulatory network analysis showed that 627 circRNAs target bound 187 miRNAs,forming 10 602 regulatory relationships.In conclusion,this study successfully screened the differentially expressed circRNAs between ivermectin sensitive and resistant strains of H.contortus,functionally annotated and enriched the source genes of the all differentially expressed circRNAs,and constructed some significant differentially expressed circRNAs-miRNAs regulatory networks.The results of this study provids a new way to study the molecular mechanism of drug resistance in H.contortus.关键词
捻转血矛线虫/伊维菌素/耐药性/转录组/circRNAs/差异分析Key words
Haemonchus contortus/Ivermectin/drug resistance/transcriptome/circRNAs/difference analysis分类
农业科技引用本文复制引用
寇慧林,王文龙,陈昕迪,王腾宇,高娃,温海峰,张艳敏,于宇,李峥,刘春霞..捻转血矛线虫耐药虫株与敏感虫株circRNAs差异表达分析[J].中国农业大学学报,2024,29(2):122-130,9.基金项目
内蒙古自然科学基金项目(2020MS03012) （2020MS03012）
"科技兴蒙"行动重点专项(NMKJXM202203-5) （NMKJXM202203-5）
