中国农业大学学报2024，Vol.29Issue(2)：131-140,10.DOI:10.11841/j.issn.1007-4333.2024.02.13
一株可裂解耐黏菌素大肠杆菌噬菌体的分离鉴定及全基因组分析
Isolation,identification and complete genomic analysis of a bacteriophage capable of lysing colistin-resistant Escherichia coli
摘要
Abstract
To study bacteriophage(phage)therapy for colistin-resistant Escherichia coli(E.coli),phage SDYTW1-F1-2-2 was isolated and purified by double AGAR plate method using colistin-resistant E.coli as host bacteria from sewage samples collected from a chicken farm in Yantai,Shandong Province.The biological characteristics of the bacteriophage were analyzed by transmission electron microscopy,optimum infection complex number,one-step growth curve,temperature stability and pH stability,and the whole genome was sequenced.The results showed as follows:1)The plaque of bacteriophage SDYTW1-F1-2-2 was transparent,halo free and had a flexible tail sheath.2)The analysis of biological characteristics showed the optimal number of phage infection was 0.1,the incubation period was 50 min,the outbreak period was 40 min,and the amount of outbreak was(331±9)PFU/cell.The phage activity was stable when the temperature was 4-40 ℃ and the pH was 4-12)The total length of the bacteriophage genome was 74752 bp,with double strands of DNA,GC content of 42.1％.A total 121 sequence coding for amino acids in protein(CDS)were identified in the genome of phage,including functional genes associated with structure composition,nucleotide metabolism and replication,packaging and bacterial lysis,a tRNA genome were discovered and no genes associated with pathogenicity genes such as stx-1 and drug resistant genes such as mcr-1 were identified.In summary,phage SDYTW1-F1-2-2 has a wide cleavage spectrum,strong acid and base tolerance,thermal stability and safety.This study provides a basis for further research on phage therapy of colistin resistant E.coli.关键词
裂解性噬菌体/耐黏菌素大肠杆菌/全基因组Key words
lytic bacteriophage/colistin-resistance E.coli/genomewide分类
农业科技引用本文复制引用
贾旭晨,肖天石,朱晓林,郝智慧..一株可裂解耐黏菌素大肠杆菌噬菌体的分离鉴定及全基因组分析[J].中国农业大学学报,2024,29(2):131-140,10.基金项目
十四五重点研发计划项目(2022YFD1801105) （2022YFD1801105）
