中国农业大学学报2024，Vol.29Issue(2)：141-151,11.DOI:10.11841/j.issn.1007-4333.2024.02.14
猪骨髓间充质干细胞成软骨分化过程能量代谢动态变化分析
Analysis of dynamic changes in energy metabolism during chondrogenic differentiation of porcine bone marrow mesenchymal stem cells
摘要
Abstract
In order to explore the dynamic changes in energy metabolism during the chondrogenic differentiation of porcine bone marrow mesenchymal stem cells(PBMSCs)and its relationship with cartilage formation,PBMSCs were isolated and identified,and porcine chondrocytes were obtained though an induction differentiation culture system.Analyses of the expression patterns of cartilage marker genes and energy metabolism-related genes were performed,as well as measurements of energy metabolites content,in PBMSCs induced to differentiate into chondrocytes for 1,6,12,and 18 days.Pearson correlation analyses between cartilage marker genes and energy metabolism products were conducted.The results showed that:1)During the chondrogenic differentiation of PBMSCs,the expression levels of cartilage marker genes,including COL2A1,SOX9,ACAN,MMP13,and RUNX2,were significantly increased(P＜0.05).The expression levels of the glycolytic rate-limiting enzyme genes HK1,PKLR,and the lactate transporter key gene SLC16A1 were significantly upregulated(P＜0.05),while the expression level of the glucose transport critical gene,SLC2A4,was significantly reduced(P＜0.05).2)With the increase of chondrogenic differentiation time,intracellular levels of ATP and glucose were significantly decreased(P＜0.05),while the intracellular ratio of lactate to glucose was significantly increased(P＜0.05).3)The correlation analysis of key chondrogenic genes and energy metabolic products suggested a significant association between the chondrogenesis process of PBMSCs and glycolytic metabolism(P＜0.05).In summary,this study successfully developed a method of inducing PBMSCs into chondrocyte,and revealed oxidative phosphorylation levels gradually decreased,and glycolytic metabolism gradually increased during the PBMSC-induced chondrogenic differentiation process.These findings provide a new perspective for further analysis of pig body length development.关键词
猪/骨髓间充质干细胞/细胞分化/软骨细胞/能量代谢Key words
pig/bone marrow mesenchymal stem cells/cell differentiation/chondrocyte/energy metabolism分类
农业科技引用本文复制引用
薛明明,李冯淼,雒亚彪,杨晓阳,谢福音,产舒恒,汤启国,方美英..猪骨髓间充质干细胞成软骨分化过程能量代谢动态变化分析[J].中国农业大学学报,2024,29(2):141-151,11.基金项目
三亚崖州湾科技城管理局2020年度科技计划项目(SKJC-2020-02-007) （SKJC-2020-02-007）
国家自然科学基金项目(32072700 （）
U22A20508) （）
国家重点研发计划(2022YFF1003401) （2022YFF1003401）
山东省重点研发计划农业良种工程(2022LZGQY007) （2022LZGQY007）
财政部和农业农村部国家现代农业产业技术体系(CARS-35) （CARS-35）
