中国农业大学学报2024，Vol.29Issue(2)：152-159,8.DOI:10.11841/j.issn.1007-4333.2024.02.15
鸭脂肪生成相关的miR-184-3p分析及关键靶基因筛选
Analysis of duck adipogenesis-related miR-184-3p and screening of key target genes
摘要
Abstract
In order to identify the key target genes of miR-184-3p affecting adipogenesis,the online databases were used to predict the miR-184-3p target genes and perform enrichment analysis of the target genes.Protein-protein interaction(PPI)networks were constructed to find the meaningful modules in the network diagram.Subsequently,the expression of target genes in adipose was analyzed,and then the key target genes were screened based on the mechanism of negative correlation between microRNA and target genes.Finally,the screening results were evaluated by RNA22 v2.The results showed that:1)A total of 225 target genes were obtained.2)GO enrichment analysis showed that target genes were involved in negative regulatory processes in cellular and macromolecular biosynthesis processes;The KEGG enrichment results contained three significant pathways,Notch signaling pathway,animal mitochondrial autophagy,and endocytosis.3)A total of 4 core genes were screened in the PPI network.4)Analysis of expression profiles screened a total of 4 genes.DVL3(Dishevelled segment polarity protein 3),HOXA9(homeobox A9),LIFR(LIF receptor subunit alpha),and SPECC1L(Sperm antigen with calponin homology and coiled-coil domains 1 like);while the RNA22 v2 evaluation showed that only LIFR had binding sites at P＜0.05.Taken together,the miR-184-3p was likely to affect duck adipogenesis by targeting DVL3,HOXA9,LIFR,and SPECC1L,with LIFR being particularly important.This study will provide a theoretical basis and data support for further study of duck miR-184-3p.关键词
鸭/miR-184-3p/脂肪生成/生物信息学Key words
duck/miR-184-3p/adipogenesis/bioinformatics分类
农业科技引用本文复制引用
金四华,贾富民,冯璐,税斐,王鑫,方毅,常亮,李欣,耿照玉..鸭脂肪生成相关的miR-184-3p分析及关键靶基因筛选[J].中国农业大学学报,2024,29(2):152-159,8.基金项目
高校优秀青年人才支持重点项目(gxyqZD2022017) （gxyqZD2022017）
安徽省教育厅高校自然科学研究重点项目(2022AH050928) （2022AH050928）
安徽省科技重大专项(201903a06020018和202103b06020015) （201903a06020018和202103b06020015）
安徽省高校协同创新项目(GXXT-2021-052) （GXXT-2021-052）
校级质量工程项目(2022yjsjy03和2022auxqhz002) （2022yjsjy03和2022auxqhz002）
高校产学研合作项目(KJ2021565和KJ2021357) （KJ2021565和KJ2021357）
教育部产学合作协同育人项目(221000488095409) （221000488095409）
