中国人兽共患病学报2023，Vol.39Issue(11)：1087-1094,1111,9.DOI:10.3969/j.issn.1002-2694.2023.00.128
胶东地区不同家禽弯曲菌流行分布及耐药特征分析
Prevalence and antimicrobial resistance of Campylobacter from poultry in the Jiaodong area
摘要
Abstract
This study was aimed to understand the current status of the antimicrobial resistance and molecular distribution of Campylobacter in various poultry in Jiaodong area,to provide a basis for effective prevention and control of the Campy-lobacter risk to poultry products and human health.Campylobacter was isolated and identified from 565 cloacal samples collect-ed in the Jiaodong area from August to October 2021 through conventional bacterial isolation and culture,mass spectrometry,microbroth dilution and multilocus sequence typing(MLST).The drug resistance and molecular typing of 131 representative strains(67 Campylobacter jejuni and 64 Campylobacter coli)were studied separately.Antimicrobial resistance analysis indica-ted that 131 isolates were highly resistant to ciprofloxacin,nalixic acid and tetracycline,with resistance rates of 96.21％,96.21％and 95.45％,respectively.Except for 2 strains,62 strains of C.coli were completely resistant to these three drugs(100％).A total of 65 strains of 131 strains were multidrug re-sistant,and the overall multidrug resistance rate was 49.62％,among which 11 strains(16.42％)of C.jejuni were resistance to 3-5 antibiotics,and 54 strains(84.38％)of C.coli were re-sistance to 3-6 antibiotics.Among the isolates from different poultry sources,waterfowl isolates were the most resistant,fol-lowed by broiler isolates.The MLST typing results revealed 72 alleles and 35 sequence types obtained from 67 strains of C.je-juni,and the distribution was relatively dispersed,without a dominant ST type and homologous complex.A total of 27 alleles and 19 sequence types were obtained from 64 strains of C.coli.Moreover,59.38％(38/64)strains were homologous complex CC-828,in which the ST-1586 sequence type was most frequent,followed by ST-825.ST-1586,ST-9944 and ST-3735 were the main sources of C.coli in broilers,and ST-825 and ST-1586 were the main sources of C.coli in waterfowl.Differences in C.jejuni and C.coli carriage were observed among poultry in the Jiaodong area.Carriage of the two bacteria was more common in laying hens than in broilers and waterfowl.C.jejuni from poultry in the Jiaodong area was highly resistant to ciprofloxacin,nalixic acid and tetracycline,but had good sensitivity to other drugs.C.coli was highly resistant to a variety of antibiotics,and multiple drug resistance was common.St-type dispersal of C.jejuni showed high genetic diversity.C.coli was cloned and transmitted mainly by ST-1586 in broiler chickens and waterfowl.Poultry carry C.jejuni,which can cause serious diseases in humans.Therefore,dynamic monitoring of Campylobacter from poultry should be strengthened.关键词
空肠弯曲菌/结肠弯曲菌/家禽/耐药性/多位点序列分型Key words
Campylobacter jejuni/Campylobacter coli/poultry/antimicrobial resistance/MLST分类
医药卫生引用本文复制引用
王娟,赵格,曲志娜,王君玮,黄秀梅,刘俊辉,李彦,刘娜,赵建梅,段笑笑,高玉斌,王琳..胶东地区不同家禽弯曲菌流行分布及耐药特征分析[J].中国人兽共患病学报,2023,39(11):1087-1094,1111,9.基金项目
青岛市科技惠民示范引导专项(No.21-1-4-ny-ll-nsh)和国家重点研发计划(No.2022YFC2303900)联合资助Supported by the Science and Technology Demonstration Guide project of Qingdao(No.21-1-4-ny-11-nsh)and National Key Re-search and Development Plan(No.2022YFC2303900) （No.21-1-4-ny-ll-nsh）
