中国人兽共患病学报2023，Vol.39Issue(11)：1100-1106,7.DOI:10.3969/j.issn.1002-2694.2023.00.130
灭活的新型结核病疫苗菌株(B/R菌株)对小鼠T淋巴细胞免疫记忆的影响
Effect of the inactivated new tuberculosis vaccine strain(B/R strain)on the immune memory of T lymphocytes in mice
摘要
Abstract
To investigate the effect of inactivated new tu-berculosis vaccine strain(B/R strain)on immune memory of T lymphocytes in mice,C57BL/6 mice were immunized with PBS,BCG strain,B/R strain and inactivated B/R strain.At week 9,12 and 15 after immunization,the spleens of each group were taken,and the spleen lymphocytes were extracted.Half of the spleen lymphocytes in each group were directly de-tected,and the rest were cultured in vitro and stimulated with PPD according to the experimental design.Flow cytometry was used to detect the number of central memory T cells(TCM)and effector memory T cells(TEM)in spleen lymphocytes of uns-timulated and stimulated mice.Without PPD stimulation after immunization,the CD4+TCM(F=13.20,P＜0.05)and CD4+TEM(F=28.15,P＜0.05)induced by inactivated B/R group,B/R group and BCG group was higher than that of PBS group at week 9.The induced CD8+TCM(F=8.92,P＜0.05)and CD8+TEM(F=6.13,P＜0.05)was higher than that of PBS group.At week 12,the CD4+TCM(F=15.97,P＜0.05)and CD4+TEM(F=13.60,P＜0.05)induced by each group was higher than that of PBS group.The induced CD8+TCM(F=5.52,P＜0.05)and CD8+TEM(F=20.15,P＜0.05)was higher than that of PBS group.At week 15,the CD4+TCM(F=15.40,P＜0.05)and CD4+TEM(F=7.43,P＜0.05)induced by each group was higher than that of PBS group.The induced CD8+TCM(F=6.57,P＜0.05)and CD8+TEM(F=9.27,P＜0.05)was higher than that of PBS group.At week 9,the CD4+TCM(F=9.66,P＜0.05)and CD4+TEM(F=11.20,P＜0.05)induced by inacti-vated B/R group,B/R group and BCG group was higher than that of PBS group.The induced CD8+TCM(F=7.24,P＜0.05)and CD8+TEM(F=9.30,P＜0.05)was higher than that of PBS group.At week 12,the CD4+TCM(F=9.33,P＜0.05)and CD4+TEM(F=6.94,P＜0.05)induced by each group was higher than that of PBS group.The induced CD8+TCM(F=67.71,P＜0.05)and CD8+TEM(F=10.86,P＜0.05)was higher than that of PBS group.At week 15,the CD4+TCM(F=39.88,P＜0.05)and CD4+TEM(F=11.93,P＜0.05)induced by each group was higher than that of PBS group.The induced CD8+TCM and CD8+TEM(F=38.47,P＜0.05)was higher than that of PBS group(F=138.80,P＜0.05).It is worth noting that at week 15,the CD8+TCM(qinactivated B/R=12.24,qB/R=12.61,P＜0.05)and CD8+TEM(qinactivated B/R=7.19,qB/R=5.00,P＜0.05)induced by inactivated B/R group and B/R group were higher than those of BCG group.The immunizing mice with inactivated B/R strain,the ability of inducing immune memory of T lymphocytes in mice was equivalent to that of B/R strain.Heat-inacti-vation did not affect the ability of B/R strain to induce immune memory in mice.关键词
结核病/灭活疫苗/T淋巴细胞/免疫记忆Key words
tuberculosis/inactivated vaccine/T lymphocytes/immune memory分类
医药卫生引用本文复制引用
丰启盈,章乐,吴芳,张万江,邓喜玲,张杰,董江涛,柳小玲,梁粟,王菊,张辉,吴江东..灭活的新型结核病疫苗菌株(B/R菌株)对小鼠T淋巴细胞免疫记忆的影响[J].中国人兽共患病学报,2023,39(11):1100-1106,7.基金项目
国家自然科学基金项目(No.82060021)、2022年度兵团指导性科技计划项目(No.2022ZD043)与石河子大学2020年度自主资助支持校级科研立项项目(No.ZZZC202013A)联合资助,丰启盈和邓喜玲对本文有同等贡献.Supported by the National Natural Science Foundation of China(No.82060021),2022 Corps guiding science and technology plan project(No.2022ZD043)and 2020 school-level scientific research project of Shihezi University(No.ZZZC202013A).Feng Qi-ying and Deng Xi-ling contributed equally to this paper. （No.82060021）
