中国蔬菜Issue(12)：42-48,7.DOI:10.19928/j.cnki.1000-6346.2023.2045
辣椒轻斑驳病毒RT-LAMP快速检测方法的建立
Establishment of a Rapid RT-LAMP Detection Method for Pepper Mild Mottle Virus
摘要
Abstract
Pepper mild mottle virus(PMMoV)is a significant viral disease that occurs in peppers.In this study,we utilized reverse transcription loop-mediated isothermal amplification technology(RT-LAMP)to develop a rapid PMMoV detection method.Specifically,we targeted the PMMoV coat protein CP sequence and designed four primers(F3/B3 and FIP/BIP).The results demonstrate this method enables specific detection of PMMoV within 60 minutes,offering a sensitivity 10 times higher than traditional RT-PCR technology.Furthermore,it does not exhibit any cross-reaction with other related viruses during the detection process.The established PMMoV RT-LAMP detection method is characterized by its simplicity,strong specificity,and high sensitivity,making it suitable for rapid detection of PMMoV in field production.关键词
辣椒轻斑驳病毒/RT-LAMP检测/快速检测Key words
PMMoV/RT-LAMP detection/rapid detection引用本文复制引用
邵煜尊,张万红,王惠,王玉洁,苗圃,申莉莉,李莹,焦裕冰,杨金广..辣椒轻斑驳病毒RT-LAMP快速检测方法的建立[J].中国蔬菜,2023,(12):42-48,7.基金项目
青岛市科技惠民示范专项(23-2-8-xdny-12-nsh),洛阳市烟草公司科技项目(2023410300200041) （23-2-8-xdny-12-nsh）
