中国生物防治学报2023，Vol.39Issue(5)：1049-1057,9.DOI:10.16409/j.cnki.2095-039x.2022.11.017
两种金小蜂类icarapin蛋白潜在致敏性的初步评价
Evaluation of the Potential Allergenicity of Icarapin-like Proteins of Two Pteromalid Wasps
摘要
Abstract
The icarapin protein of Western honey bee,Apis mellifera,is a common human allergen and its homologues also exist in parasitoid wasps.In order to evaluate the potential allergenicity of icarapin-like proteins in parasitoid wasps,the icarapin-like protein encoding genes from Pteromalus puparum and Nasonia vitripennis were successfully cloned and expressed.Sequence analysis showed that the open reading frames of icarapin-like genes in the two parasitoid wasps were 654 bp and 714 bp,respectively,and both were predicted to be potentially allergenic.The recombinant icarapin-like proteins were purified by Ni-ion affinity chromatography.Using the BALB/c mouse model for potential allergenicity assessment,we found that the concentrations of histamine and immunoglobulin E(IgE)in mice serum injected with recombinant proteins of the two parasitoid wasps and A.mellifera were higher than those of the negative control group injected with PBS,indicating that icarapin-like proteins from the two pteromalid wasps have potential allergenicity.This study provides a reference for the pre-application safety assessment of parasitoid bioactive proteins.关键词
蝶蛹金小蜂/丽蝇蛹集金小蜂/icarapin蛋白/表达与纯化/致敏性评价Key words
Pteromalus puparum/Nasonia vitripennis/icarapin-like protein/expression and purification/allergenicity assessment分类
农业科技引用本文复制引用
曹昀珅,杨义,方琦,汪芳,叶恭银..两种金小蜂类icarapin蛋白潜在致敏性的初步评价[J].中国生物防治学报,2023,39(5):1049-1057,9.基金项目
国家自然科学基金(31830074) （31830074）
