中国生物防治学报2023，Vol.39Issue(5)：1083-1093,11.DOI:10.16409/j.cnki.2095-039x.2022.03.030
全局调控子MtrAsbh影响米尔贝霉素生物合成的研究
Effect of the Global Regulator MtrAsbh on the Biosynthesis of Milbemycins
摘要
Abstract
MtrA is a global response regulatory protein that is highly conserved in Streptomyces.The function of MtrA from Streptomyces bingchenggensis(MtrAsbh)in the biosynthesis of biopesticide milbemycins was studied by by gene deletion,complementation,overexpression,transcriptional analysis and gel retardation assay.Results showed that the mtrAsbh deletion mutant displayed a complete loss of milbemycin production,while overexpression of mtrAsbh significantly promoted the production of milbemycin,indicating that MtrAsbh is a key activator of milbemycin biosynthesis.Our results further show that MtrAsbh regulates milbemycin production by affecting the expression of milbemycin biosynthetic gene clusters and genes related to precursor supply.In addition,MtrAsbh can act as an activator or a repressor to differentially control the expression of many other natural product biosynthesis genes presented in the genome of Streptomyces bingchenggensis.关键词
全局调控子/MtrAsbh/冰城链霉菌/米尔贝霉素Key words
global regulator/MtrAsbh/Streptomyces bingchenggensis/milbemycin分类
农业科技引用本文复制引用
祝亚杰,王佳彬,李珊珊,向文胜,张艳艳..全局调控子MtrAsbh影响米尔贝霉素生物合成的研究[J].中国生物防治学报,2023,39(5):1083-1093,11.基金项目
国家自然科学基金(31872936) （31872936）
