中国生物防治学报2023，Vol.39Issue(5)：1094-1103,10.DOI:10.16409/j.cnki.2095-039x.2022.11.020
ganA在路德维希肠杆菌杀松材线虫中的功能解析
Functional Analysis of ganA in the Nematocidal of Enterobacter ludwigii against Bursaphelenchus xylophilus
摘要
Abstract
Biocontrol microorganisms have been used to control Bursaphelenchus xylophilus disease,but the molecular mechanism of nematicidal is not clear.This study found that the corrected mortality of B.xylophilus treated with E.ludwigii AA4 was more than 85%,and strain AA4 could significantly reduce B.xylophilus infection of pine seedlings.In order to explore the molecular mechanism of AA4 against B.xylophilus,the DNA homologous recombination technique was used to construct the β-galactosidase gene ganA,and the function of ganA in AA4 nematode inhibition was analyzed.The results showed that the B.xylophilus killing activity and B.xylophilus disease prevention in a ganA knockout mutant strain decreased significantly,and the mobility and ability to colonize nematodes of the mutant strain decreased significantly.In addition,compared with the wild-type strain,the ability of the mutant strain to restrict the movement of nematodes was significantly reduced.Therefore,ganA may participate in the process of AA4 killing B.xylophilus by regulating AA4's ability to colonize B.xylophilus and restrict nematode movement.关键词
基因ganA/路德维希肠杆菌/松材线虫/杀线活性/定殖Key words
ganA/Enterobacter ludwigii/Bursaphelenchus xylophilus/nematocidal activity/colonization分类
农业科技引用本文复制引用
王浩宇,李海勇,王爽,牛犇,张弘弢,张莹,赵宇,王传珍,张丽霞,王琦,吴迪,于翠芳..ganA在路德维希肠杆菌杀松材线虫中的功能解析[J].中国生物防治学报,2023,39(5):1094-1103,10.基金项目
国家重点研发计划(2022YFD1401900) （2022YFD1401900）
北京市公园管理中心科技课题(zx2021002) （zx2021002）
黑龙江省自然科学基金(YQ2019C002) （YQ2019C002）
国家自然科学基金(32071741) （32071741）
