中国生物防治学报2023，Vol.39Issue(5)：1104-1112,9.DOI:10.16409/j.cnki.2095-039x.2023.01.041
一株球孢白僵菌的分离鉴定及其对茶丽纹象甲幼虫的室内致病力测定
Isolation,Identification,and Pathogenicity of a Beauveria bassiana Strain Isolated from Tea Weevils Myllocerinus aurolineatus Larvae
摘要
Abstract
Tea weevil is an important tea insect pest in Chinese tea plantations,currently chemical pesticides are the main control method and environmentally friendly control strategies are needed urgently.To screen potential pathogenic strains for biological control of tea weevils,an entomopathogenic fungi strain isolated from field-collected infected dead Myllocerinus aurolineatus larvae was studied.Morphological characteristics and phylogenetic tree analysis revealed that the isolated strain was Beauveria bassiana(Bals.)Vuill,herein named as Maure 1.1.Under the natural pupating and hatching temperature(22℃),the laboratory pathogenicity increased with the prolonged post-inoculation observation period when M.aurolineatus larvae were exposed to B.bassiana Maure 1.1 at a concentration of 1×105―1×108 conidia/mL.When applied at the highest concentration,the Maure 1.1 strain led to 76.67%and 73.33%of cumulative corrected mortalities with immersion and soil mixing methods,respectively,at 11 days post-treatment.The LC50 were 2.00×103 and 1.11×103 conidia/mL,and the LT50 were 7.87 and 7.84 days for the two methods,respectively.These results indicate that the isolated B.bassiana Maure 1.1 strain is biologically pathogenic to tea weevils'larvae and a potential candidate strain for M.aurolineatus biocontrol.关键词
茶丽纹象甲/球孢白僵菌/分离鉴定/致病力测定/生物防治Key words
Myllocerinus aurolineatus/Beauveria bassiana/identification/pathogenicity/biological control分类
农业科技引用本文复制引用
付楠霞,罗宗秀,李兆群,边磊,修春丽,陈宗懋,蔡晓明..一株球孢白僵菌的分离鉴定及其对茶丽纹象甲幼虫的室内致病力测定[J].中国生物防治学报,2023,39(5):1104-1112,9.基金项目
国家重点研发计划(2021YFD1601100)) （2021YFD1601100）
国家现代农业产业技术体系建设专项资金(CARS-19) （CARS-19）
