中国生物防治学报2023，Vol.39Issue(5)：1148-1155,8.DOI:10.16409/j.cnki.2095-039x.2022.05.009
褐飞虱RNAi显微注射中麻醉方法的比较
Comparison of Anesthesia Methods in RNAi Microinjection of Brown Planthopper
摘要
Abstract
Microinjection is an effective and accurate quantitative method to inhibit gene expression,which has a wide range of applications in insect gene function research and pest control.Usually,the tested insects are small and vigorous,and they need to be anesthetized before injection for immobilization.After the anesthesia effect is relieved,further phenotypic determination experiments can be performed.Different anesthesia methods have different effects on the anesthesia effect,physiology,and behavior of insects.Therefore,the determination of insect anesthesia methods is of great significance to reduce errors in microinjection dsRNA interference experiments.In this study,the brown planthopper was used as the experimental subject,and the anesthetic effects of different proportions of ether and ethyl acetate mixed liquid anesthetics and low temperature anesthesia on the brown planthopper were compared,and the effects on the survival rate of brown planthopper were further determined.The results showed that the recovery time of brown planthopper was prolonged with the increase of the concentration of ethyl acetate in anesthetic treatment.The effects of different anesthesia methods on the survival rate of brown planthopper in the RNAi microinjection experiment ranked from high to low were ether:ethyl acetate(1:2),ether:ethyl acetate(1:1),ether,ether:ethyl acetate(2:1),ether:ethyl acetate(3:1),20 min on ice,and 40 min on ice.The comprehensive evaluation showed that the mixture of ether:ethyl acetate(1:2)could be used as the optimal anesthesia treatment for the RNAi microinjection experiment of brown planthopper.关键词
褐飞虱/液体麻醉/低温麻醉/显微注射/RNA干扰Key words
brown planthopper/liquid anesthesia/hypothermic anesthesia/microinjection/RNA interference分类
农业科技引用本文复制引用
张朋稳,段至柔,刘常权,彭英传,张万娜,肖海军,张晶..褐飞虱RNAi显微注射中麻醉方法的比较[J].中国生物防治学报,2023,39(5):1148-1155,8.基金项目
江西省自然科学基金(20212BAB21501) （20212BAB21501）
江西省教育厅自然科学基金(9020308488) （9020308488）
