中国生物防治学报2023，Vol.39Issue(5)：1235-1243,9.DOI:10.16409/j.cnki.2095-039x.2023.02.051
两种芽胞杆菌对苗期土传辣椒疫病的防控作用
Prevention and Control Effect of Two Bacillus Strains on Soil-borne Phytophthora capsici in Seedling Stage
摘要
Abstract
Transplanting seedlings with pathogen results in the spread of pepper blight.In order to effectively use biocontrol bacterium to prevent and control pepper blight at seedling stage,the efficacy of Bacillus velezensis LYK10 and Bacillus cereus QTK2 against pepper blight of seedling by soaking seed were tested by point plate seedling with biocontrol bacteria.The synergies of mixed biocontrol bacterium and biocontrol bacteria combined chemical fungicide were mensurated.The results showed that LYK10 and QTK2 could delay the occurrence time of pepper seedling disease,and the control efficiency of pepper seedling disease was respectively 30.55%and 33.33%,and the emergence rate was increased by 1.39%and 2.78%in turn.The both bacterium had antagonistic effects on Phytophthora capsici.,and also induced the changes of CAT and PPO enzyme activity in pepper plants.The efficiency of the two mixed bacterium at equal concentration improved from 27.78%to 42.45%compared with the two single bacteria treatments.The efficiency of the mixture of LYK10 and dienomorph with the consistence of 5.0×107 cfu/mL+300 µg/mL and 5.0×106 cfu/mL+150 µg/mL respectively were 48.15%and 38.89%.The control effect of high concentration bacterial-drug treatment was higher than the treatments of 108 cfu/mL LYK10 and 300 g/mL dienomorph.The control effect of low concentration bacterial-drug treatment was higher than LYK10 and comparable to dienomorph.The results can provide data to support for the research on biological control to pepper blight and effective utilization of biocontrol bacterium.关键词
辣椒疫病/育苗期/芽胞杆菌/生防菌混合使用/菌药联用Key words
Phytophthora capsici/seedling stage/Bacillus sp./mixed bacterium/bacterial-drug mixture分类
植物保护学引用本文复制引用
祝文慧,秦溥,赵岩,殷辉,任璐,周建波..两种芽胞杆菌对苗期土传辣椒疫病的防控作用[J].中国生物防治学报,2023,39(5):1235-1243,9.基金项目
山西省重点研发计划(201803D221005-7) （201803D221005-7）
山西省现代农业产业技术(蔬菜)体系建设 （蔬菜）
山西省农业科学院农业科技创新研究课题(YCX2020426) （YCX2020426）
