中国输血杂志2023，Vol.36Issue(12)：1085-1089,5.DOI:10.13303/j.cjbt.issn.1004-549x.2023.12.001
新冠病毒抗体ELISA筛查假阳性反应:不同方法检测结果的内在逻辑
False positive reaction of COVID-19 antibody ELISA screening:internal logic of various methods
摘要
Abstract
Objective To analyze the results of different methods for reactive samples screened by the enzyme linked immunosorbent assay(ELISA)against severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)in blood donors.Methods From March to April 2020,a total of 8 632 blood samples in Shenzhen were screened for SARS-CoV-2 total anti-bodies(TAb,including IgG,IgM,IgA)in plasma using ELISA(PC group),the antibody reactivity samples and their follow up plasma samples(FC group),and samples of disease control group(DC group)from January to April 2020 were detected using the following methods:1)ELISA method for detecting IgG,IgM,and(or without detection)TAb;2)pseudovirus neutralizing antibody test(pVNT);3)western blot(WB)of SARS-CoV-2 antibody.The negative control group(NC group)from February to April 2020 performed ELISA and WB testing.Results Among the 34 total antibody positive samples,2 were positive for pVNT test,and the total antibody,IgG and WB in the initial screening and tracking testing were positive.Thereaft-er,it was determined to be confirmed positive.The other 2 cases were positive for pVNT test,while the samples with positive WB results were in the follow-up stage.The TAb,IgG,and pVNT results did not conform to the dynamic evolution of antibodies,and cannot be determined as confirmed positive.Conclusion The infection status of antibody reactivity samples screened by SARS-CoV-2 ELISA can be judged by the logic of pVNT,WB and the dynamic change of antibody.关键词
新型冠状病毒/反应性标本/假病毒中和抗体试验/免疫印迹试验/动态监测Key words
severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)/antibody reactivity samples/pseudovirus neutralizing antibody test/Western blot(WB)/dynamic monitoring分类
医药卫生引用本文复制引用
王立林,李然,李彤,张爱佳,刘衡,许晓绚,曾劲峰,邬林枫..新冠病毒抗体ELISA筛查假阳性反应:不同方法检测结果的内在逻辑[J].中国输血杂志,2023,36(12):1085-1089,5.基金项目
2020年度广东省医学基金项目(A2020259) （A2020259）
深圳市医学重点学科建设经费(SZXK070) （SZXK070）
深圳市医疗卫生三名工程项目(SZSM2023028) （SZSM2023028）
深圳市血液中心科研课题(SZBC202202) （SZBC202202）
