中国输血杂志2023，Vol.36Issue(12)：1102-1106,5.DOI:10.13303/j.cjbt.issn.1004-549x.2023.12.005
4种静注人免疫球蛋白制品的自身IgG抗体多样性研究
Diversity of IgG autoantibodies of 4 Chinese intravenous immunoglobulins
摘要
Abstract
Objective To develop methods to display the IgG autoantibody repertoire of intravenous immunoglobulin(IVIG)products,analyze the different types of antibodies and study the diversity of IgG autoantibody in 4 IVIG preparations from different Chinese manufacturers.Methods Two-dimensional gel electrophoresis and immunoblotting with human um-bilical vein endothelial cell(HUVEC)proteins were used to demonstrate the IgG autoantibody repertoire and the human pro-tein microarray with bioinformatics analysis was employed to profile the immune reactive autoantigens of the 4 IVIG prepara-tions.Results The methods to showcase the autoantibody repertoire and study the antibody diversity of IVIG were success-fully established.High-quality repertoires of IVIG autoantibodies and biological information about self-proteins that can be recognized were obtained.There was a significant difference in the recognition of the quantity and variety of the self-antigens by different IVIG products.The number of antibodies against HUVEC proteins in four products ranged from 241-386.The number of proteins recognized on the human protein chip ranged from 292-435,with 172 human self-proteins recognized by all four products.Conclusion Demonstration of antibody repertoire and protein chip technology can be used to analyze IVIG products′IgG autoantibody repertoire.All four preparations tested in this study exhibited a broad spectrum of antibodies a-gainst HUVEC proteins and human proteome microarray,each product had its unique antibody repertoire characteristics.关键词
静注人免疫球蛋白/自身IgG抗体/抗体谱/抗体多样性Key words
intravenous immunoglobulin(IVIG)/IgG autoantibody/antibody repertoire/diversity of antibodies分类
医药卫生引用本文复制引用
晏晓晨,马莉,王宗奎,李长清,雷婷婷,杜晞,叶生亮..4种静注人免疫球蛋白制品的自身IgG抗体多样性研究[J].中国输血杂志,2023,36(12):1102-1106,5.基金项目
四川省科技计划(2023ZHCG0066,2023NSFSC0714),四川省医学青年创新科研课题(Q22009),中国医学科学院医学健康与创新工程(2021-I2M-060) （2023ZHCG0066,2023NSFSC0714）
