中国输血杂志2023，Vol.36Issue(12)：1110-1114,5.DOI:10.13303/j.cjbt.issn.1004-549x.2023.12.007
献血者HBsAg阴性NAT可疑标本HBV感染状态确认
Confirmation of HBV infection of HBsAg-negative NAT suspicious sample from blood donors
摘要
Abstract
Objective To explore the HBV infection of initially reactive but discriminatory test non-reactive(NAT sus-picious)samples of voluntary blood donors after PANTHER individual nucleic acid testing(ID-NAT)in Tianjin.Methods From January to August 2021,after routine testing and PANTHER ID-NAT,a total of 66 HBsAg-NAT reactive but dis-criminatory test non-reactive samples(referred to as NAT suspicious samples)were tested from 69 362 blood samples.Among which,23 samples were selected by simple random sampling method and enriched by ultra-high speed centrifugation.HBV DNA was detected by supersensitive fluorescence quantification PCR(qPCR)and ID-NAT,and electrochemiluminescence was supplemented for two and half pairs of hepatitis B detection.Results Among 23 suspicious NAT samples,14 were con-firmed HBV DNA positive by serological and molecular biological tests,and the anti-HBc positive rate of HBV infected indi-viduals was 92.8%.92.8%(13/14)of the infected individuals were occult hepatitis B virus infection(OBI).A total of 10 samples were detected for viral load by qPCR,of which5 were quantifiable,with viral load of(11～464)IU/mL and a me-dian of 15.4 IU/mL.Conclusion 60%of the NAT suspicious samples were detected as HBV DNA positive.Anti-HBc tes-ting can exclude most OBI undetectable by NAT,and the sensitivity of NAT should be improved to ensure the safety of blood transfusion.关键词
单人份核酸检测/隐匿性乙型肝炎病毒感染/荧光定量/电化学发光法Key words
ID-NAT/OBI/fluorescent quantitation/electrochemiluminescence分类
医药卫生引用本文复制引用
王全慧,樊晶..献血者HBsAg阴性NAT可疑标本HBV感染状态确认[J].中国输血杂志,2023,36(12):1110-1114,5.
