摘要
Abstract
Objective To investigate the changes in complement content in component blood before blood transfusion.Methods 180 samples from 20 plasma donors were collected at different stages of the preparation process,stored at differ-ent temperatures and time periods,and tested in the same batch to observe the changes in complement C3 and C4 levels un-der different storage temperatures and process stages.Results The same sample was used to test C3 and C4 levels,and the test results were sorted into nine groups for comparison according to different storage temperatures and preparation process stages.C3:0.994 1;C4:0.957 1,with no significant difference in storage at-40℃and-60℃(P＞0.05);C3:0.133 3,0.224 06(P＞0.05);C4:0.027 3,0.025 1(P＜0.05),storing at 4℃for 7 days may significantly reduce complement levels,which needs further verification;C3:0.047 0,0.038 3;C4:0.042 6,0.012 1(P＜0.05),virus inactivation preparation process can significantly reduce complement levels,repeated freeze-thawing during frozen plasma processing can change com-plement levels.The correlation analysis of C3 and C4 content determination experiment shows that they are significantly posi-tively correlated under different storage temperature,preparation process and storage time(P＜0.05).Conclusion Storage temperature,storage time,preparation process of frozen plasma such as repeated freeze-thawing,centrifugal concentration and light inactivation have an impact on complement content.关键词
补体含量/血浆/温度/保存条件Key words
complement content/plasma/temperature/storage conditions分类
医药卫生
