中国输血杂志2023，Vol.36Issue(12)：1162-1164,3.DOI:10.13303/j.cjbt.issn.1004-549x.2023.12.020
一例RhD--缺失型患者的血清学及分子生物学分析
Serological and molecular biological analysis of RhD--:a case report
摘要
Abstract
Objective To study the blood group serology and molecular biology of patients with RhD--,so as to guide clinical blood use.Methods The EDTA-K2 anticoagulant blood of the patient was detected for Rh antigens and antibodies.Meanwhile,DNA was extracted,and the 1-10 exon of RHCE and RHAG gene was sequenced by Sanger sequencing.Results The serological test showed O type RhD--,and all spectral cells were positive.RHCE gene sequencing showed RHCE∗02/RHCE∗02,RHAG gene sequencing showed mutations at site 808 G＞A and site 861 G＞A of exon 6.Conclusion When patients were with RhD--,and related immune conditions such as pregnancy and/or transfusion history were present,autologous blood transfusion or plasma exchange could be an option for emergency blood use.关键词
Rh表现型/血清学试验/基因测序/RHCE/RHAGKey words
Rh phenotype/serological test/gene sequencing/RHCE/RHAG分类
医药卫生引用本文复制引用
水波,侯治兵,姚丽,严威,何继武..一例RhD--缺失型患者的血清学及分子生物学分析[J].中国输血杂志,2023,36(12):1162-1164,3.基金项目
襄阳市科技计划指导性项目(2022BBS005204) （2022BBS005204）
