中国兽医杂志2023，Vol.59Issue(11)：1-10,10.
SGK通过下调抑癌基因p27蛋白促进小鼠肿瘤生长
SGK Promotes Tumor Growth in Mice via Inhibiting Tumor Suppressor Gene p27 Protein
摘要
Abstract
Tumor has become a common disease in elderly animals.Serum and glucocorticoid-induced protein kinase(SGK)is highly expressed in various malignant tumors,but its mechanism in tumor growth has not been fully elucidated.To investigate the impact and mechanism of SGK on tumor growth,this study used the human breast cancer cell line(MDA-MB-231)and knocked down SGK using small interfering RNA(siRNA).The effects of SGK on MDA-MB-231 cell proliferation and transformation were assessed using XTT,bromodeoxyuridine(BrdU),and soft agar colony formation assays.Mouse embryonic fibroblast cell lines NIH3T3/HER2/SGK and NIH3T3/HER2/SGK/p27 were constructed using an overexpression system.XTT,BrdU,flow cytometry,and soft agar colony formation assays were employed to investigate whether SGK regulates tumor cell proliferation,transformation,and cell cycle through the anti-cancer gene p27 protein.The mechanism of SGK regulation of p27 was analyzed using Western blot(WB)and immunofluorescence(IF)techniques.A mouse xenograft tumor model was established,and tumor volume calculation and immunohistochemistry(IHC)were used to analyze the mechanism of SGK regulation of tumor growth.The results showed that knocking down SGK significantly reduced tumor cell proliferation and transformation(P＜0.05).Overexpression of p27 markedly decreased tumor cell proliferation and transformation(P＜0.05),and the number of cells in the G0-G1 phase increased,which was reversed by SGK overexpression.Additionally,SGK overexpression induced the cytoplasmic localization of p27.In the mouse tumor model,SGK decreased p27 protein levels and promoted tumor growth.These findings suggest that SGK can promote tumor cell growth by inhibiting p27 both in vitro and in vivo,indicating SGK as a potential diagnostic and prognostic indicator for animal tumor diseases and a promising target for cancer drug development.关键词
小鼠/肿瘤/血清和糖皮质激素诱导的蛋白激酶/p27Key words
mice/tumor/serum and glucocorticoild-induced protein kinase/p27分类
农业科技引用本文复制引用
安一娜,谭姝瑜,刘天龙,许建海,董彦君..SGK通过下调抑癌基因p27蛋白促进小鼠肿瘤生长[J].中国兽医杂志,2023,59(11):1-10,10.基金项目
国家自然科学基金项目(3177130338) （3177130338）
