中国兽医杂志2023，Vol.59Issue(11)：37-41,5.
钩吻提取物对猪肝脏药物代谢酶活性的影响
Effects of Gelsemium elegans Extracts on Activities of Drug-Metabolizing Enzymes in Pig Liver
摘要
Abstract
To investigate the impact of Gelsemium elegans extracts on the activities of cytochrome P450(CYP450)and NAD(P)H:quinone oxidoreductase 1(NQO1)in pig liver,this study utilized a 95％ethanol solution to prepare Gelsemium elegans alcohol extract and a 0.5％sulfuric acid solution to prepare Gelsemium elegans water extract.Fifty-six-day-old piglets were randomly divided into five groups,each consisting of 15 piglets:T1 group(basic diet),T2 group(basic diet+150 μg/mL Gelsemium elegans water extract),T3 group(basic diet+25 μg/mL Gelsemium elegans alcohol extract),T4 group(basic diet+50 μg/mL Gelsemium elegans alcohol extract),and T5 group(basic diet+100 μg/mL Gelsemium elegans alcohol extract).The piglets were continuously fed for 47 days,and liver samples were collected to prepare microsomes and cytosol.The impact of Gelsemium elegans extracts on the activities of aminopyrine-N-demethylase(AND)and erythromycin-N-demethylase(ERND)in liver microsomes was analyzed using a colorimetric method.Additionally,the effect of Gelsemium elegans extracts on NQO1 activity in liver cytosol was investigated.The results showed that,compared with the T1 group,T2 group had no significant effect on AND and ERND activities(P＞0.05),while T3 and T4 groups had an inducing effect on both activities(P＜0.05 orP＜0.01).Both extracts significantly inhibited NQO1 activity(P＜0.01).These findings suggest that Gelsemium elegans alcohol extract induces CYP450 activity,while Gelsemium elegans extracts exhibit inhibitory effects on NQO1 activity.This study elucidates the impact of Gelsemium elegans on drug-metabolizing enzymes,indicating potential interactions when co-administered with other drugs and providing pharmacological evidence for the further clinical application of Gelsemium elegans.关键词
钩吻/细胞色素P450(CYP450)/醌氧化还原酶(NQO1)/诱导/抑制Key words
Gelsemium elegans/cytochrome P450(CYP450)/NAD(P)H:quinone oxidoreductase 1(NQO1)/induction/inhibition分类
农业科技引用本文复制引用
孟思雨,张卓亿,伍勇,孙志良,刘兆颖..钩吻提取物对猪肝脏药物代谢酶活性的影响[J].中国兽医杂志,2023,59(11):37-41,5.基金项目
国家自然科学基金(31972737) （31972737）
