中国兽医杂志2023，Vol.59Issue(11)：42-46,5.
产细菌素乳酸菌LS-1的全基因组测序分析
Whole Genome Sequencing Analysis of Bacteriocin-Producing Lactic Acid Bacteria LS-1
摘要
Abstract
In previous research,a lactic acid bacterium Lacticaseibacillus saniviri(LS-1)capable of antagonizing Staphylococcus aureus was isolated from handmade cheese in Inner Mongolia.To explore the complete genome information of LS-1 and uncover potential bacteriocin-encoding gene clusters,this study conducted whole genome sequencing of LS-1,predicted coding genes,and annotated them using the non-redundant protein(Nr)and gene ontology(GO)databases.The BAGEL4 database was used to predict and analyze bacteriocin synthesis gene clusters.The results revealed that the genome size of LS-1 was 2 356 714 bp with a GC content of 47.87％.A total of 2 316 coding genes were predicted,with a total length of 2 058 534 bp and an average length of 888 bp.The secreted bacteriocin from LS-1 belongs to the Enterolysin A(EnlA)of Class Ⅲ bacteriocins,suggesting that EnlA might exert bactericidal activity by lysing the cell wall.This study comprehensively analyzed the LS-1 genome through whole-genome sequencing,elucidating the bacteriocin-encoding gene clusters at the genetic level,and further confirming the potential of LS-1-produced bacteriocin as a novel antimicrobial product.关键词
细菌素/乳酸菌/Lacticaseibacillus saniviri/全基因组测序分析/基因簇Key words
bacteriocin/lactic acid bacteria/Lacticaseibacillus saniviri/whole genome sequencing analysis/gene cluster分类
农业科技引用本文复制引用
涂会鑫,董文龙..产细菌素乳酸菌LS-1的全基因组测序分析[J].中国兽医杂志,2023,59(11):42-46,5.基金项目
吉林农业科技学院博士启动基金项目(20215021) （20215021）
