中国兽医杂志2023，Vol.59Issue(11)：47-51,5.
鲤鱼双线绦虫裂头蚴的分子鉴定和系统发育关系分析
Molecular Identification and Phylogenetic Relationships Analysis of Digramma interuota in Cyprinus carpio
摘要
Abstract
To identify the"noodle-like"worm parasites found in the abdominal cavities of common carp from a fish farm in Tianjin,PCR was used to amplify the 18S rRNA,CO Ⅰ,and COB gene sequences of the worms.Molecular identification and gene sequencing were performed,and the homology,molecular evolution,and phylogenetic relationships based on these three genes were analyzed.The results indicated that the 18S rRNA,CO Ⅰ,and COB gene sequences of the worms parasitizing the Cyprinus carpio showed high homology with nucleotide sequences from Digramma interuota isolates MN204040.1(China),MN219466.1(China),and EU241209.1(France),with homologies of 99.45％,98.74％,and 99.26％,respectively.Phylogenetic trees constructed based on the 18S rRNA,CO Ⅰ,and COB gene sequences showed that the tapeworm plerocercoid isolated in this study clustered in the same branch as the Digramma interuota species identified in the GenBank database,and they were closely related to the Digramma interuota isolate from Wuhan,China.This study provides initial insights into the phylogenetic relationship of the Digramma interuota isolate obtained in Tianjin with other species,supporting molecular identification and phylogenetic analysis of Digramma interuota.关键词
双线绦虫/舌状绦虫/分子鉴定/系统发育Key words
Digramma interuota/Ligula intestinalis/molecular identification/phylogeny分类
农业科技引用本文复制引用
许晓琳,张旻,景宏丽,陈冬杰,王慧煜,袁向芬,孔玉方,吕继洲,吴绍强..鲤鱼双线绦虫裂头蚴的分子鉴定和系统发育关系分析[J].中国兽医杂志,2023,59(11):47-51,5.基金项目
中国检验检疫科学研究院基本科研业务费项目(2022JK43,2023JK003) （2022JK43,2023JK003）
