中国兽医杂志2023，Vol.59Issue(11)：52-58,7.
枯草芽孢杆菌肽聚糖对绵羊瘤胃上皮细胞β-防御素-1(SBD-1)表达的影响
Effects of Bacillus subtilis Peptidoglycan on Expression of β-defensin-1(SBD-1)in Ovine Rumen Epithelial Cells
摘要
Abstract
To explore the influence of peptidoglycan derived from Bacillus subtilis cell walls on the expression of β-defensin-1(SBD-1)in ovine rumen epithelial cells(ORECs),this study stimulated ORECs with 30 μg/mL peptidoglycan at different durations(2,4,6,8,10,and 12 h).The mRNA and protein expression levels of SBD-1 were detected using quantitative fluorescence PCR(qPCR)and enzyme-linked immunosorbent assay(ELISA),respectively,to determine the optimal stimulation time.Subsequently,ORECs were stimulated with different concentrations of peptidoglycan(0,10,20,30,40,and 50 μg/mL)for 2 h,and the mRNA and protein expression levels of SBD-1 were detected using qPCR and ELISA to determine the optimal stimulation concentration.The results showed that:(1)When ORECs were stimulated with 30 μg/mL peptidoglycan for different durations,the mRNA and protein expression levels of SBD-1 initially decreased and then increased with time,with the highest expression at 2 h,significantly higher than the control group(P＜0.001).(2)When ORECs were stimulated with different concentrations of peptidoglycan for 2 h,the mRNA and protein expression levels of SBD-1 increased initially and then decreased with the concentration of peptidoglycan,reaching the highest expression at 20 μg/mL,significantly higher than the control group(P＜0.001).(3)Stimulation experiments at different times and different concentrations did not induce cytotoxicity in ORECs.The results indicate that peptidoglycan derived from Bacillus subtilis can induce the expression of SBD-1 in ORECs,with the optimal stimulation condition being 20 μg/mL peptidoglycan from Bacillus subtilis for 2 h.关键词
枯草芽孢杆菌肽聚糖/绵羊瘤胃上皮细胞(ORECs)/β-防御素-1(SBD-1)/荧光定量PCR(qPCR)/酶联免疫吸附试验(ELISA)/CCK8Key words
Bacillus subtilis peptidoglycan/ovine rumen epithelial cells(ORECs)/β-defensin-1(SBD-1)/quantitative fluo-rescence PCR(qPCR)/enzyme-linked immunosorbent assay(ELISA)/CCK8分类
农业科技引用本文复制引用
齐盟,辛雅明,白绥明,胡亮,杨银凤..枯草芽孢杆菌肽聚糖对绵羊瘤胃上皮细胞β-防御素-1(SBD-1)表达的影响[J].中国兽医杂志,2023,59(11):52-58,7.基金项目
国家自然科学基金项目(32060780) （32060780）
内蒙古自治区教育厅项目(NJZZ21015) （NJZZ21015）
