中国兽医杂志2023，Vol.59Issue(11)：59-68,10.
生猪屠宰过程沙门菌污染状况及其耐药性传播风险
Salmonella Contamination and Drug Resistance Transmission Risk in Pig Slaughtering Process
刘云哲 1刘俊辉 2耿国芹 3王君玮 2邹明 4赵格 2赵建梅 2王琳 2杨腾腾 2高玉斌 2黄秀梅 2张青青 2张喜悦2
作者信息
- 1. 中国动物卫生与流行病学中心致病微生物监测室,农业农村部畜禽产品质量安全风险评估实验室(青岛),山东青岛 266032||青岛农业大学动物医学院,山东青岛 266109
- 2. 中国动物卫生与流行病学中心致病微生物监测室,农业农村部畜禽产品质量安全风险评估实验室(青岛),山东青岛 266032
- 3. 临沂市畜牧技术推广站,山东临沂 276000
- 4. 青岛农业大学动物医学院,山东青岛 266109
- 折叠
摘要
Abstract
This study aims to reveal the spread and drug resistance of Salmonella in the process of pork production by monitoring the contamination distribution and drug resistance of Salmonella in each link of pig slaughter and pork products.Five representative pig slaughterhouses in Shandong province were selected as risk monitoring sites.A total of 552 samples,including anal swabs,environmental swabs,water samples,and carcass swabs,were collected.Salmonella isolates were isolated and identified using the national food safety standards and Matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF MS).The drug sensitivity of Salmonella isolates was determined using the microbroth dilution method.Multilocus sequence typing(MLST)was used to identify the genotypes of Salmonella.The results showed that a total of 58 Salmonella strains were isolated,with a total positive detection rate of 10.5％(58/552).Salmonella contamination was more severe in small and medium-scale slaughterhouses(15.6％,33/212)than in large-scale slaughterhouses(7.4％,25/340).The highest detection rate was observed in the samples collected in pre-cooling stage of the slaughtering process,with a rate of 26.7％(8/30).Among different types of samples,the highest detection rate was in the anal swab samples,with a rate of 15.1％(16/106).The highest drug resistance rate was observed for ampicillin(96.6％,56/58),and the multi-drug resistance rate was 96.6％(56/58).Salmonella isolates from small and medium-scale slaughterhouses exhibited more severe overall drug resistance.The multi-drug resistance rate was higher in carcass swabs(46.4％,26/56)and anal swab samples(28.6％,16/56)than in environmental swab samples(25.0％,14/56).MLST gene typing revealed that the 58 Salmonella isolates corresponded to 6 ST types,with ST40 being the most widespread and detected in all stages,followed by ST19.The results indicate that Salmonella contamination is severe during the pig slaughtering process,highlighting the need to strengthen hygiene control during slaughter.Pig slaughterhouses of all scales should pay attention to the transmission of drug-resistant bacteria and genes in the industry and food chains,and develop reasonable prevention and control measures to prevent potential food safety risks.关键词
生猪屠宰/沙门菌/污染风险/耐药传播/分子分型Key words
pig slaughtering/Salmonella/contamination risk/drug resistance transmission/molecular typing分类
农业科技引用本文复制引用
刘云哲,刘俊辉,耿国芹,王君玮,邹明,赵格,赵建梅,王琳,杨腾腾,高玉斌,黄秀梅,张青青,张喜悦..生猪屠宰过程沙门菌污染状况及其耐药性传播风险[J].中国兽医杂志,2023,59(11):59-68,10.
