中国兽医杂志2023，Vol.59Issue(11)：69-73,5.
鸡胚源大肠杆菌的分离鉴定及其致病性和耐药性分析
Isolation,Identification,Pathogenicity and Drug Resistance Analysis of Escherichia coli from Chicken Embryo
摘要
Abstract
In order to understand the pathogenicity and drug resistance of Escherichia coli(E.coli)from chicken embryos,this study isolated E.coli from the yolk and embryonic feces of 174 randomly sampled unhatched dead embryos from six different batches of eggs in a broiler hatchery in Shandong Province.The strains were isolated and identified using bacterial culture,morphological observation,and biochemical tests.The pathogenicity of the isolated E.coli strains was determined through mouse inoculation tests and PCR detection of virulence genes.The drug resistance of the isolates was assessed by the paper disk diffusion method.The results showed that 28 strains of E.coli were isolated and identified.In the mouse inoculation test,27 E.coli isolates had a mortality rate over 50.0％,with 24 strains exceeding 83.0％.Among the 14 tested virulence genes,papA,papC,eaeA,and vat were not detected,while fimC had the highest detection rate(85.7％),and the detection rates of the other nine virulence genes ranged from 3.6％to 64.3％.There was a positive correlation between the pathogenicity of the strains and the number of carried virulence genes.Drug resistance testing showed that E.coli isolates had varying degrees of sensitivity to 10 antibiotics,with resistance rates ranging from 17.9％to 89.3％.The results indicate that majority of the isolated E.coli from chicken embryos are pathogenic,showing different degrees of resistance to commonly used clinical antibiotics.This study provides reference information for the management and control of vertical transmission of E.coli in breeder farms and the selection of antibiotics in clinical practice.关键词
鸡胚/大肠杆菌/致病性/毒力基因/耐药性Key words
chicken embryo/Escherichia coli(E.coli)/pathogenicity/virulence gene/drug resistance分类
农业科技引用本文复制引用
汤朝晖,赵健超,任慧英,张灿,邹玲,尹燕博,刘宗柱,刘文华..鸡胚源大肠杆菌的分离鉴定及其致病性和耐药性分析[J].中国兽医杂志,2023,59(11):69-73,5.基金项目
山东省现代农业产业技术体系家禽产业创新团队项目(SDAIT-011-03) （SDAIT-011-03）
山东省重点研发计划项目(2019GNC106058) （2019GNC106058）
