中国兽医杂志2023，Vol.59Issue(11)：132-139,8.
火炭母通过调控TLR4-TBK1信号通路改善鼠伤寒沙门菌感染小鼠空肠的炎症反应
Polygonum chinense L.Alleviated Jejunum Inflammatory Response in Mice Infected by Salmonella typhimurium through Regulating TLR4-TBK1 Signaling Pathway
摘要
Abstract
In order to investigate the immunoprotective effects of Polygonum chinense L.(PCL)on the jejunum of mice infected with Salmonella typhimurium and its potential mechanisms,48 female BALB/c mice were randomly divided into six groups:Blank control group,Model group,Positive drug group,and High-,Medium-,and Low-dose PLC groups,with 8 mice in each group.Mice in the Positive drug group were orally administered with gentamicin[20 mg/(kg·bw)],and mice in the High-,Medium-,and Low-dose PLC groups were orally administered with PLC at doses of 16,8,and 4 g/(kg·bw),respectively.Mice in the Blank control group and Model group were given an equal volume of sterile saline,with a total of 8 days of continuous administration.After 2 days of prophylactic treatment,each mouse(excluding the Blank control group)was orally challenged with 0.2 mL of half-lethal dose(LD50)of Salmonella typhimurium(5 x 104 CFU/mL)to induce infection.After completion of the treatment,the mice were euthanized,and the jejunum was collected for histopathological examination and Western blot.The results showed that following infection with Salmonella typhimurium,the mice in the Model group exhibited increased goblet cells in jejunum and erythrocytes in intestinal gland,and loose structure of intestinal villi.Mice in the PLC groups showed significant reduction of goblet cells in jejunum and erythrocytes in intestinal gland,indicating that PLC alleviated intestinal tissue damage in mice.Western blot results demonstrated that compared with the Blank control group,the Model group exhibited significant increase in interferon-β(IFN-β)protein expression(P＜0.05),significant decrease in interferon-γ(IFN-γ)protein expression(P＜0.05),and significant upregulation of interferon regulatory factor 3(IRF3),phosphorylated TANK-binding kinase 1(p-TBK1),and Toll-like receptor 4(TLR4)protein expression(P＜0.05).Additionally,inflammatory factors interleukin-6(IL-6),interleukin-1 β(IL-1β),and nuclear factor kappa B p65(NF-κB p65)showed significant increase in expression(P＜0.05).In comparison to the Model group,different doses of PLC reduced the expression of inflammatory factors IL-6(P＜0.05 or P＞0.05),IL-1β(P＜0.05),and NF-κB p65(P＜0.05),increased the expression of TLR4,interferon-β TIR domain-containing adaptor protein(TRIF),TANK-binding kinase 1(TBK1),and its phosphorylation levels(P＜0.05),as well as the downstream signaling protein IRF3(P＜0.05)and its phosphorylation levels(P＜0.05 or P＞0.05).This led to increase in IFN-β(P＜0.05 or P＞0.05)and IFN-γ(P＜0.05)protein expression in the jejunum.The results suggested that PCL alleviated jejunum inflammatory response in mice infected by Salmonella typhimurium through regulating TLR4-TBK1 signaling pathway.关键词
火炭母/鼠伤寒沙门菌/空肠/Toll样受体4(TLR4)/TANK结合激酶1(TBK1)Key words
Polygonum chinense L/Salmonella typhimurium/jejunum/Toll like receptor 4(TLR4)/TANK binds kinase 1(TBK1)分类
农业科技引用本文复制引用
张易安,郭琰娜,刘妍,刘涵笑,沈幸玲,刘逸雷,唐陆平,何永明..火炭母通过调控TLR4-TBK1信号通路改善鼠伤寒沙门菌感染小鼠空肠的炎症反应[J].中国兽医杂志,2023,59(11):132-139,8.基金项目
广东省省级科技计划项目(2015A040404048) （2015A040404048）
广东省农业农村厅项目(2019KJ119) （2019KJ119）
广东省教育厅项目(2017GCZX006) （2017GCZX006）
