中国现代医学杂志2023，Vol.33Issue(23)：1-9,9.DOI:10.3969/j.issn.1005-8982.2023.23.001
PLA2G4和ABCC1基因多态性与支气管哮喘患儿孟鲁司特疗效的研究
Study on the polymorphisms of PLA2G4 and ABCC1 genes and the efficacy of montelukast in children with bronchial asthma
摘要
Abstract
Objective To investigate the correlation between single nucleotide polymorphisms(SNPs)of cytosolic phospholipase A2(PLA2G4)gene and ATP-binding cassette subfamily C member 1(ABCC1)gene and the efficacy of leukotriene receptor antagonists(LTRA)montelukast in children with bronchial asthma.Methods A total of 121 asthmatic children treated at the Northern Theater General Hospital of the People's Liberation Army from March 2019 to September 2021 were selected for the study.The children received routine treatment plus montelukast for one month.The imLDRTM technique was used to detect SNPs of PLA2G4 gene at rs10157410,rs10489409,rs932476 loci,and ABCC1 gene at rs215066 locus.Changes in lung function,inflammatory indicators,and asthma symptom control levels were explored among different genotypes at each locus before and after treatment.Results The comparison of lung function indicators among different genotypes of PLA2G4 gene at rs10157410,rsl0489409,and rs932476 loci before and after montelukast treatment showed no significant differences(P＞0.05).After montelukast treatment,the percentage of predicted forced expiratory volume in 1 second(FEV1),the ratio of FEV1 to forced vital capacity(FVC),and the percentage of predicted peak expiratory flow at 50％and 75％of vital capacity significantly increased for genotypes GC and GG at rs10157410 locus,CT and TT at rs 10489409 locus,GA and AA at rs932476 locus,and for genotype CC at ABCC1 gene rs215066 locus(P＜0.05).After montelukast treatment,there was no significant improvement in eosinophil count in children with genotype CC at rs10489409 locus(P＞0.05),while immunoglobulin E(IgE)and exhaled nitric oxide levels significantly improved for different genotypes at PLA2G4 and ABCC1 gene loci compared to pre-treatment(P＜0.05).After montelukast treatment,the proportion of good control for genotype GC at rs10157410 locus was significantly higher than GG,and for genotypes GA and AA at rs932476 locus,the proportion of good control was significantly different from GG(P＜0.05).Logistic regression analysis showed that the proportion of good control for genotype GC at PLA2G4 gene rs10157410 locus was 5.639 times that of GG[OR=5.639(95％CI:2.078,15.298)],and the proportion of good control for genotype GG at rs932476 locus was 0.053 times that of AA[OR=0.053(95％CI:0.006,0.430)].Conclusion PLA2G4 gene rs10157410 genotype GC and rs932476 genotype AA have better sensitivity to the efficacy of montelukast.关键词
支气管哮喘/儿童/基因多态性/胞浆型磷脂酶A2/ATP结合盒亚家族C成员1/孟鲁司特Key words
bronchial asthma/children/genetic polymorphism/cytosolic phospholipase a2/ATP-binding cassette subfamily C member 1/montelukast分类
医药卫生引用本文复制引用
任梦洋,魏兵,李令雪,贾京晶,张世楠,尤瑄,支艳杰..PLA2G4和ABCC1基因多态性与支气管哮喘患儿孟鲁司特疗效的研究[J].中国现代医学杂志,2023,33(23):1-9,9.基金项目
辽宁省民生科技计划(No:2021JH2/10300060) （No:2021JH2/10300060）
沈阳市科技计划(No:20-205-4-062) （No:20-205-4-062）
