中国现代医学杂志2023，Vol.33Issue(23)：61-66,6.DOI:10.3969/j.issn.1005-8982.2023.23.011
不同温度凝胶对大鼠寒区皮肤放射损伤修复的研究
Effect of different temperature hydrogel on repairing radiation-induced skin injury of SD rats in cold region
摘要
Abstract
Objective To investigate the effects of different temperature gels on the repair of cold-region skin radiation injury.Methods Twenty SD rats were randomly divided into a 4 ℃ gel group and a 37 ℃ gel group.After one week of rearing in a-20 ℃ low-temperature environment,a cold-region skin radiation injury model in SD rats was replicated by strontium-90 irradiation on both sides of the back for 10 minutes.The skin repair was observed at different time points after injury,and histopathological changes were observed using hematoxylin-eosin(HE)staining.Western blotting was performed to detect the expression of vascular endothelial growth factor(VEGF),alpha-smooth muscle actin(α-SMA),collagen Ⅰ,and collagen Ⅲ.Enzyme-linked immunosorbent assay(ELISA)was used to measure platelet-derived growth factor D(PDGFD)and transforming growth factor-beta(TGF-β)levels.Results There was no significant difference in body weight between the 4 ℃ gel group and the 37 ℃ gel group(P＞0.05).Compared with self-control,rats showed skin damage after irradiation,presenting as erythema and ulceration.The severity of skin damage increased first and then gradually decreased over time,with the most severe damage observed on day 21 after irradiation.After gel intervention at different temperatures,the degree of skin damage in rats as reduced at different time points.Compared with self-control,the 37 ℃ gel group showed the most significant promotion of wound healing.HE staining results showed that the epidermis thickened,and the dermis was infiltrated with lymphocytes after irradiation.Gel intervention reduced skin tissue damage,and epithelial recovery was most pronounced in the 37 ℃ gel group.Western blotting results showed that the relative expression levels of VEGF,α-SMA,Collagen Ⅰ,and Collagen Ⅲ proteins in skin tissues of rats in the self-control,4℃ gel,and 37℃ gel groups were statistically different(P＜0.05).After gel intervention,the relative expression levels of VEGF,α-SMA,Collagen Ⅰ,and Collagen Ⅲ proteins in skin tissues of rats in the 37 ℃ gel group were significantly increased.ELISA results showed that the levels of PDGFD and TGF-β in skin tissues of the 37 ℃ gel group were significantly increased(P＜0.05).Conclusion Under low-temperature conditions,strontium-90 irradiation can cause skin damage in rats.A 37 ℃ gel is more beneficial for the healing of cold-region skin radiation injury compared to a 4 ℃ gel.关键词
皮肤放射损伤/大鼠/寒区/凝胶Key words
skin radiation injury/rats/cold region/gel分类
医药卫生引用本文复制引用
刘颖,王思潼,王秋..不同温度凝胶对大鼠寒区皮肤放射损伤修复的研究[J].中国现代医学杂志,2023,33(23):61-66,6.基金项目
军队护理创新与培育专项计划(No:2021HL057) （No:2021HL057）
辽宁省自然科学基金(No:2023-BS-032) （No:2023-BS-032）
