中国畜牧兽医2023，Vol.50Issue(12)：4868-4875,8.DOI:10.16431/j.cnki.1671-7236.2023.12.009
烟酰胺对泌乳期奶牛瘤胃乳酸代谢及其相关酶活力的影响
Effects of Nicotinamide on Rumen Lactate Metabolism and Related Enzyme Activities in Lactating Dairy Cows
摘要
Abstract
[Objective]This study was aimed to investigate the effect of nicotinamide(NAM)on rumen fermentation status and lactate metabolism and its related enzyme activity in lactating dairy cows,and provide data reference for nicotinamide regulating rumen carbohydrate metabolism.[Method]Forty Holstein dairy cows in good body condition,with similar lactation age(170 d± 50 d),litter size(2.23±0.62),milk yield(36.17 kg±7.40 kg)and health,were randomly divided into control and experiment groups(NAM7,NAM11 and NAM15),with 10 dairy cows in each group.The cows in control group were fed the basal diet,while the cows in NAM7,NAM11 and NAM15 groups were supplemented with 7,11 and 15 g/d nicotinamide in the basic diet,respectively.The experiment period lasted for a total of 75 d,including 15 d for the pre-test period and 60 d for the trial period.Rumen fluid was collected before morning feeding on days 30 and 60 of the trial period,and the levels of volatile fatty acids such as acetic acid,propionic acid and butyric acid,and the concentrations of pyruvic acid,malic acid and succinic acid in rumen fluid were determined by high performance gas chromatography.The lactic acid,pyruvate kinase and lactate dehydrogenase were determined by spectrophotometer.[Result]① The rumen fermentation efficiency of dairy cows in three experiment groups were extremely significantly higher than that in control group(P＜0.01),among which,the rumen fermentation efficiency of dairy cows in NAM7 group was extremely significantly higher than that in NAM11 and NAM15 groups(P＜0.01).② The concentrations of rumen pyruvate,malate and succinate in NAM7 group were significantly higher and the lactic acid concentration in NAM7 group was significantly lower than that in control group(P＜0.05).③The NAD+concentration in three experiment groups were significantly higher than that in control group(P＜0.05),and the NAD+concentration in NAM7 group was extremely significantly higher than that in NAM11 and NAM15 groups(P＜0.01).The activities of rumen pyruvate kinase,malate dehydrogenase and succinate dehydrogenase in NAM7 group were significantly higher than that in control group(P＜0.05),and the NADH concentration and lactate dehydrogenase activity were significantly lower than that in control group(P＜0.05).[Conclusion]Nicotinamide could improve rumen fermentation efficiency,the concentrations of NAD+,pyruvic acid,malic acid and succinic acid,and the activities of pyruvate kinase,malate dehydrogenase and succinate dehydrogenase in Holstein dairy cows,reduce the concentrations of lactic acid and NADH,and lactate dehydrogenase activity in rumen.The recommended nicotinamide addition rate was 7 g/d.关键词
烟酰胺/泌乳期奶牛/瘤胃发酵/乳酸/乳酸代谢相关酶Key words
niacinamide/lactating dairy cows/rumen fermentation/lactic acid/lactate metabolism related enzyme分类
农业科技引用本文复制引用
刘拓,院东,王永力,努热耶·吾斯曼,臧长江,成志强,刘建成,南冰禹,李凤鸣,李云梦,仇娅琪..烟酰胺对泌乳期奶牛瘤胃乳酸代谢及其相关酶活力的影响[J].中国畜牧兽医,2023,50(12):4868-4875,8.基金项目
新疆维吾尔自治区重大科技专项"高产奶牛均衡与精准营养技术集成示范"(2020A01001) （2020A01001）
