中国畜牧兽医2023，Vol.50Issue(12)：4925-4934,10.DOI:10.16431/j.cnki.1671-7236.2023.12.015
不同月龄滩羊肌肉组织转录组比较及脂肪酸分析
Transcriptome Comparison and Fatty Acid Analysis of Muscle Tissue in Tan Sheep at Different Ages
摘要
Abstract
[Objective]The differentially expressed genes of fat metabolism in 4-and 9-month-old Tan sheep were compared in this study,and the genes affecting fat metabolism in different muscle tissues of Tan sheep were excavated.[Method]Longissimus dorsi muscle and biceps femoris muscle of 4-and 9-month-old Tan sheep were collected,the transcriptomic sequencing was performed by Illumian software,and the fat and fatty acid contents of longissimus dorsi muscle and biceps femoris muscle were analyzed.[Result]Compared with 4-month-old Tan sheep,the fat content in biceps femoris muscle of 9-month-old Tan sheep was significantly decreased(P＜0.01),the fat content in longissimus dorsi muscle was significantly increased(P＜0.01),the unsaturated fatty acid contents such as oleic acid were increased,and the saturated fatty acid contents such as myristic acid and palmitic acid were decreased.Transcriptome sequencing results showed that a total of 893 differentially expressed genes(435 up-regulated and 458 down-regulated)were screened in biceps femoris muscle,and 809 differentially expressed genes(544 up-regulated and 265 down-regulated)were screened in longissimus dorsi muscle.GO function enrichment analysis showed that the differentially expressed genes in biceps femoris muscle of Tan sheep were significantly enriched to 327 entries,which were related to fat deposition,such as TCA cycle,respiratory chain complex,hydrogen ion transmembrane transporter activity and so on;28 terms were significantly enriched in longissimus dorsi muscle,including organic acid catabolic metabolism,fatty acid metabolism,extracellular space and so on.KEGG pathway annotation results showed that the differentially expressed genes in biceps femoris muscle of Tan sheep were significantly enriched to 25 pathways,9 of which were involved in fat metabolism,such as TCA cycle,pyruvate metabolism and so on;8 pathways were significantly enriched in longissimus dorsi muscle,2 of which were involved in fat deposition,namely,degradation of valine,leucine and isoleucine and glutathione metabolism.[Conclusion]By screening the genes related to fat metabolism pathways,MDH2 and PPARGC1A genes in biceps femoris muscle and GSTK1 and MCCC2 genes in longissimus dorsi muscle might be related to fat metabolism in muscles of 9-month-old Tan sheep.The results provided a theoretical reference for further research on fat deposition in muscle tissue of Tan sheep at different growth stages.关键词
滩羊/转录组/脂肪酸/背最长肌/股二头肌Key words
Tan sheep/transcriptome/fatty acid/longissimus dorsi muscle/biceps femoris muscle分类
畜牧业引用本文复制引用
杜佳文,任文义,康燕,徐晓锋,张力莉..不同月龄滩羊肌肉组织转录组比较及脂肪酸分析[J].中国畜牧兽医,2023,50(12):4925-4934,10.基金项目
国家自然科学基金(31660668) （31660668）
