中国畜牧兽医2023，Vol.50Issue(12)：4973-4982,10.DOI:10.16431/j.cnki.1671-7236.2023.12.019
沐川乌骨黑鸡LIPE基因第1外显子多态性及其与体重、肌内脂肪含量的关联分析
Polymorphism of LIPE Gene Exon 1 and Its Association with Body Weight and Intramuscular Fat Content in Muchuan Black-bone Chickens
摘要
Abstract
[Objective]The aim of this study was to investigate the genetic variation of single nucleotide polymorphism(SNP)of hormone-sensitive lipase(LIPE)gene exon 1 in Muchuan Black-bone chickens,and analyzed the correlation with body weight and intramuscular fat(IMF)content.[Method]The blood of 106 Muchuan Black-bone chickens was collected,and the SNP site of LIPE gene exon 1 was detected by PCR amplification and direct sequencing.SPSS 21.0 was used to analyze the SNP polymorphism of LIPE gene exon 1 and its correlation with body weight and IMF content of chest muscle in Muchuan Black-bone chickens.The effect of SNP of LIPE gene exon 1 on its amino acid was analyzed by bioinformatics online tool.Real-time quantitative PCR was used to detect the relative expression of LIPE gene in different tissues and chest muscles of different genotypes of Muchuan Black-bone chickens.[Result]A total of 3 SNP sites were detected of LIPE gene exon 1 in Muchuan Black-bone chickens.Genotypes of AA,AC and CC were found in SNP1(c.66 A＞C).Genotypes of AA and AG were found in SNP2(c.440 A＞G).Genotypes of GG and GA were found in SNP3(c.462 G＞A).Chi-square fitness test showed that SNP2 and SNP3 of LIPE gene exon 1 in Muchuan Black-bone chickens were in Hardy-Weinberg equilibrium(P＞0.05),and SNP1 deviated from Hardy-Weinberg equilibrium(P＜0.05).Association analysis showed that the IMF content in chest muscle of AG genotype with SNP2 locus was significantly higher than that of AA genotype(P＜0.05),and there was no significant difference in body weight among different genotypes with each locus(P＞0.05).Amino acid sequence alignment analysis showed that SNP2 was a non-synonymous mutation,which caused lysine to become arginine.Real-time quantitative PCR results showed that the expression level of LIPE gene was higher in adipose tissue,and the mRNA expression level of LIPE gene in chest muscle of AA genotype with SNP2 locus was extremely significantly higher than that of AG genotype(P＜0.01).[Conclusion]There are three SNP of LIPE gene exon 1 in Muchuan Black-bone chickens,among which SNP2 locus has a significant effect on IMF content in Muchuan Black-bone chickens,and it could be used as a candidate molecular genetic marker for IMF content selection in Muchuan Black-bone chickens.关键词
沐川乌骨黑鸡/LIPE基因/第1外显子/肌内脂肪/遗传变异Key words
Muchuan Black-bone chickens/LIPE gene/exon 1/intramuscular fat/genetic variation分类
农业科技引用本文复制引用
苟丹妮,王钢,沈雪梅,聂亮,惠文静,古丽帕日·艾克拜,杨雅玲,廖娟,喻世刚..沐川乌骨黑鸡LIPE基因第1外显子多态性及其与体重、肌内脂肪含量的关联分析[J].中国畜牧兽医,2023,50(12):4973-4982,10.基金项目
四川省科技计划资助-重点研发项目(2022YFN0039) （2022YFN0039）
大学生创新创业训练计划项目(S202210649100、202110649014) （S202210649100、202110649014）
乐山师范学院科技计划项目(DGZZ202002、22HX00051) （DGZZ202002、22HX00051）
