中国畜牧兽医2023，Vol.50Issue(12)：5084-5093,10.DOI:10.16431/j.cnki.1671-7236.2023.12.030
1株H1N1亚型猪流感病毒的进化分析及分子特征研究
Evolutionary Analysis and Molecular Characteristics of a H1N1 Subtype Swine Influenza Virus
摘要
Abstract
[Objective]The purpose of this experiment was to identify the circulating strain of Swine influenza virus(SIV)and its molecular biological characteristics in Guizhou province,and provide important reference for the etiological research and epidemiological investigation of swine influenza.[Method]70 nasal swabs of pigs were collected from some farms in Guizhou province,identified by RT-PCR and inoculated with canine kidney epithelial cells(MDCK)for virus isolation and identification.The whole genome sequence of the strain was obtained by cloning and sequencing.The genetic recombination and key amino acid molecular characteristics of the strain were analyzed by bioinformatics online software.[Result]The strain of H1N1 subtype SIV was isolated and named A/swine/Guizhou/ZY/2022(H1N1).Whole genome sequencing and genetic evolution analysis showed that this strain belonged to G4 type Eurasian avian like H1N1 SIV.Bioinformatics online prediction showed that HA and NA genes of the isolated strain belonged to Eurasian avian like H1N1 branch,NS gene belonged to North American recombinant branch,and the rest of the gene fragments belonged to the 2009 pandemic HfN1 branch.The cleavage site of HA protein was PSIQSR ↓ GL,there were 4 potential glycosylation sites,and the receptor binding sites and antigenic sites were highly consistent with Eurasian avian like H1N1 SIV.The NA protein was structurally intact with 5 potential glycosylation sites.There were Q591 R and R251 K mutations in PB2 protein,N375 S,L473 V and S31 N mutations in PB1 protein and M2 protein,respectively.[Conclusion]A 3-source rearranged H1N1 subtype SIV strain was isolated from Guizhou province,which belonged to G4 type Eurasian avian like H1N1 virus,and its binding ability to human sialic acid α2,6-GAL receptor might be enhanced,suggesting to strengthen the epidemiological surveillance of swine influenza.关键词
猪流感病毒/H1N1亚型/遗传进化/分子特征Key words
Swine influenza virus/H1N1 subtype/genetic evolution/molecular characteristics分类
农业科技引用本文复制引用
黄书,潘向英,田红利,曾智勇,梁海英,汤德元,王彬,边孟婷,柳佳佳,张婧旭,万娟..1株H1N1亚型猪流感病毒的进化分析及分子特征研究[J].中国畜牧兽医,2023,50(12):5084-5093,10.基金项目
贵州省科技支撑计划项目(黔科合支撑L2021]一般162) （黔科合支撑L2021]一般162）
