中国畜牧兽医2023，Vol.50Issue(12)：5114-5122,9.DOI:10.16431/j.cnki.1671-7236.2023.12.033
金定鸭神经症状相关致病菌分离和鉴定
Isolation and Identification of Pathogenic Bacteria Associated with Neurological Signs of Jinding Ducks
摘要
Abstract
[Objective]The aim of the present study was to determine the pathogenic bacteria responsible for neurologic signs of Jinding ducks.[Method]Twenty-two sick ducks,which displayed neurologic signs and lesions of fibrinous pericarditis,perihepatitis and airsacculitis,were randomly selected from four 24-to 48-day-old flocks of Jinding ducks from a region with intensive egg-laying duck production in Liaoning province.Brain tissues were sampled and bacterial isolation were performed by using tryptic soy agar(TSA)and MacConkey's agar(MCA),and identified by Gram and Wright's staining,biochemical testing,and 16S rDNA gene sequencing.[Result]The 19 strains of bacteria isolated from the brain tissue of Jinding duck were all Gram-negative bacteria.Among them,17(named H1-H17)failed to grow in MCA and tested negative in the biochemical assays,whereas other 2 isolates(named H18 and H19)formed red colonies in MCA,fermented glucose,lactose,and maltose,and were methyl red and indole positive.On the basis of comparative analyses of the 16S rDNA amplicon sequences,the 17 isolates represented by H1 shared 99.1％-100％identities with reference strains of RA serotypes 1 to 19.The H18 and H1 9 isolates were 99.2％-99.9％identical to the selected eight reference Escherichia coli strains.Phylogenetic analysis based on the amplified 16S rDNA amplicon sequences further demonstrated that the 17 isolates represented by H1 belonged to RA and that isolates H18 and H19 belonged to Escherichia coli.[Conclusion]In the studied cases,RA was responsible for neurologic signs in Jinding ducks,and that Escherichia coli could also cause neurologic signs in Jinding ducks.关键词
金定鸭/鸭疫里默氏菌/大肠杆菌/神经症状Key words
Jinding duck/Riemerella anatipestifer/Escherichia coli/neurologic signs分类
农业科技引用本文复制引用
刘贺,李琼,黄晶晶,胡晓阳,王笑言,赵辉..金定鸭神经症状相关致病菌分离和鉴定[J].中国畜牧兽医,2023,50(12):5114-5122,9.基金项目
国家现代农业产业技术体系(CARS-42-16、CARS-42-32) （CARS-42-16、CARS-42-32）
