中国畜牧兽医2023，Vol.50Issue(12)：5170-5185,16.DOI:10.16431/j.cnki.1671-7236.2023.12.038
基于网络药理学探讨黄芩提取物防治猪链球菌2型感染的作用机制
Exploring the Mechanism of Scutellaria baicalensis Georgi Extract in Preventing and Treating Streptococcus suis Type 2 Infection Based on Network Pharmacology
摘要
Abstract
[Objective]The aim of this study was to explore the potential mechanism of Scutellaria baicalensis Georgi extract in preventing and treating Streptococcus suis type 2(SS2)infection.[Method]The transcriptome data of SS2-infected cells were collected by GEO database,and differentially expressed genes were screened by GEO2R online software.The protein-protein interaction(PPI)inetwork was constructed using STRING database and Cytoscape v3.8.2 software,and topological analysis was performed.The changes of core protein,biological function and signal transduction pathway after SS2 infection were screened by GO function and KEGG pathway enrichment analysis.Liquid chromatography mass spectrometry(LC-MS)was used to analyze and identify the components of Scutellaria baicalensis Georgi extract,and Swiss ADME and SwissTargetPredication databases were used to screen the active components of Scutellaria baicalensis Georgi extract and predict its target.The mechanism of anti-SS2 infection of Scutellaria baicalensis Georgi extract was further defined by combining the disease target with the active ingredient target by network pharmacology.Molecular docking and Real-time quantitative PCR were used to verify the feasibility of the network pharmacological prediction results.[Result]1 308 differential genes of SS2 infection were identified by two sets of gene chips provided by GEO database,and 70 core genes were screened by PPI analysis.GO functional enrichment analysis showed that significant enrichment items mainly involved cytokine-mediated signaling pathways and responses to cytokine stimulation.KEGG pathway enrichment analysis involved the TNF,PI3K-Akt,JAK-STAT and other signal transduction pathways.A total of 31 active components of Scutellaria baicalensis Georgi extract,including baicalin and baicalin,etc.,which might affect core targets such as VEGFA,TNF and PPARG,involving multiple biological functions and signaling pathways such as inflammatory response,apoptosis and promotion of angiogenesis.Molecular docking results showed that baicalin and baicalin of Scutellaria baicalensis Georgi extract had good binding energy with TNF,PPARG and VEGFA.Real-time quantitative PCR results showed that the mRNA levels of CA9,PPARG,JUN,SCD,JAK3 and VEGFA genes in PAMs cells were significantly up-regulated after SS2 infection(P＜0.05),and the mRNA expression levels of these genes were significantly decreased after the intervention of Scutellaria baicalensis Georgi extract(P＜0.05).[Conclusion]After SS2 infection,cytokine mediated biological processes,TNF and other signaling pathways changed and the active components of Scutellaria baicalensis Georgi extract might jointly participate in apoptosis regulation,inflammatory response,angiogenesis and other biological processes through AMPK,PI3K-Akt,GFR and TNF signaling pathways,thereby preventing SS2 infection.关键词
猪链球菌2型/黄芩提取物/液质联用/网络药理学/分子对接Key words
Streptococcus suis type 2/Scutellaria baicalensis Georgi extract/LC-MS/network pharmacology/molecular docking分类
农业科技引用本文复制引用
刘佳丽,杨小林,闫普普,刘恋,朱君,夏瑾瑾,黄永熙,白若男,郭利伟,刘国平..基于网络药理学探讨黄芩提取物防治猪链球菌2型感染的作用机制[J].中国畜牧兽医,2023,50(12):5170-5185,16.基金项目
湖北省重点研发计划项目(2023BBB045) （2023BBB045）
