中国畜牧兽医2023，Vol.50Issue(12)：5186-5193,8.DOI:10.16431/j.cnki.1671-7236.2023.12.039
表没食子儿茶素-3-没食子酸酯缓解顺铂所致大鼠急性肾损伤效果评价
Effect of Epigallocatechin Gallate on Alleviating Acute Kidney Injury Induced by Cisplatin in Rats
摘要
Abstract
[Objective]This study was aimed to evaluate the protective effects of different concentrations of epigallocatechin gallate(EGCG)on cisplatin-induced acute kidney injury in rats,and to select the best concentration of EGCG to alleviate cisplatin nephrotoxicity.[Method]Thirty Wistar rats were randomly divided into 5 groups:Normal control group(CON),cisplatin group(CIS),low dose(20 mg/kg)EGCG+cisplatin group(LCE),medium dose(40 mg/kg)EGCG+cisplatin group(CE)and high dose(80 mg/kg)EGCG+cisplatin group(HCE).Rats in CON group and CIS group were perfused with normal saline(40 mg/kg)for 28 days,and CIS group was intraperitoneally injected with CIS(7 mg/kg)on the 26th day.Rats in LCE,MCE and HCE groups were given corresponding dose of EGCG for 28 days,CIS was injected intraperitoneally on the 26th day,and serum and kidney tissues were taken on the 29th day.Firstly,the changes of physiological and biochemical indexes of rats were detected during drug administration,then the pathological changes of kidney tissue of rats in each group were detected,the protective effect of EGCG on acute kidney injury induced by CIS was preliminarily evaluated,and the best effective concentration of EGCG was selected.Then the protein expression changes of apoptosis(Bax,Bcl-2 and Caspase-3)and inflammation(IL-6 and IL-10)were detected by Western blotting to further confirm the protective effect of EGCG.[Result]Compared with CON group,the diet,drinking water and body weight of rats in CIS group decreased significantly on the 28th day,while urea nitrogen(BUN)and creatinine(CRE)increased significantly(P＜0.05).The renal tubules were obviously dilated,with protein cast,and the renal injury was serious.Compared with CIS group,the diet,water consumption and weight of rats in MCE group were increased significantly(P＜0.05),and the contents of BUN and CRE in serum were decreased significantly(P＜0.05).The renal tubular dilatation in MCE group was significantly improved,but there was no significant change in LCE and HCE groups.Therefore,40 mg/kg EGCG was selected for the follow-up experiment.Western blotting result showed that compared with CON group,the expressions of Bax,IL-6 and Caspase-3 in CIS group were increased significantly(P＜0.05),while the expressions of Bcl-2 and IL-10 decreased significantly(P＜0.05).Compared with CIS group,the expressions of Bax,IL-6 and Caspase-3 in MCE group were decreased significantly(P＜0.05),while the expressions of Bcl-2 and IL-10 were increased significantly(P＜0.05).[Conclusion]EGCG had a protective effect on CIS-induced acute kidney injury in rats(40 mg/kg was the best),which was related to the inhibition of renal cell apoptosis and inflammation.关键词
表没食子儿茶素-3-没食子酸酯(EGCG)/顺铂/大鼠/急性肾损伤Key words
epigallocatechin gallate(EGCG)/cisplatin/rat/acute kidney injury分类
农业科技引用本文复制引用
汪星萍,曹家慧,苟丹丹,邵义文,迟士鹏,杨春雪,徐恩爽..表没食子儿茶素-3-没食子酸酯缓解顺铂所致大鼠急性肾损伤效果评价[J].中国畜牧兽医,2023,50(12):5186-5193,8.基金项目
2022年大学生创新创业训练项目(国家级一般项目)(202210223045) （国家级一般项目）
