中国畜牧兽医2023，Vol.50Issue(12)：5204-5212,9.DOI:10.16431/j.cnki.1671-7236.2023.12.041
2株金黄色葡萄球菌耐药性分析及中药对其部分耐药基因表达的影响
Drug Resistance Analysis of 2 Staphylococcus aureus Strains and the Influence of Traditional Chinese Medicine on Expression of Some Drug Resistance Genes
摘要
Abstract
[Objective]The purpose of this experiment was to study and determine the degree of resistance of Staphylococcus aureus to common antibiotics and traditional Chinese medicine,and to explore the influence of traditional Chinese medicine on the expression of resistance genes in Stabhylococcus aureus.[Method]The resistance of Stabhvlococcus aureus SA1 and SA2 to commonly used antibiotics was detected by Kirby-Bauer method.The minimum inhibitory concentrations(MIC)of 10 traditional Chinese medicines was determined,and the 4 traditional Chinese medicines with the best effect were selected.The strains were added to BHI medium containing 3％glucose at different concentrations of 4 traditional Chinese medicine(0,1/2,1/4 and 1/8 MIC),and the effects of 4 traditional Chinese medicines on the expression of some drug resistance genes in Staphylococcus aureus were detected by Real-time quantitative PCR.[Result]The results of drug sensitivity test showed that SA1 strain was highly resistant to ampicillin,moderately resistant to ceftazidime,and sensitive to other drugs.SA2 strain had high resistance to ampicillin,penicillin,erythromycin,clindamycin,vancomycin and oxacillin,and sensitive to other drugs.The results of MIC determination of 2 strains of Staphylococcus aureus by 10 kinds of traditional Chinese medicine decoction showed that Coptis chinensis,Cortex Phellodendri chinensis,Galla chinensis and Sanguisorba officinalis L.had the best inhibitory effect on 2 strains.Real-time quantitative PCR results showed,under different liquid concentrations,Galla chinensis significantly decreased the expression of mecA and mecB genes in SA1 strain(P＜0.01).Coptis chinensis and Cortex Phellodendri chinensis significantly decreased mecB gene expression in SA1 strain(P＜0.01).Coptis chinensis and Sanguisorba officinalis L.significantly decreased mecA gene expression in SA2 strain(P＜0.01).[Conclusion]Traditional Chinese medicine could inhibit Staphylococcus aureus and reduce the expression level of related drug resistance genes to a certain extent,among which Coptis chinensis,Galla chinensis and Sanguisorba officinalis L.have the best effect,followed by Cortex Phellodendri chinensis.The results provided a reference for the prevention and treatment of Sta phylococcus aureus with traditional Chinese medicine.关键词
金黄色葡萄球菌/耐药性/中药/耐药基因Key words
Staphylococcus aureus/drug resistance/traditional Chinese medicine/resistance gene分类
畜牧业引用本文复制引用
张亚倩,王亚男,郭上朝,张洪伟,史秋梅,蒋海龙,赵希艳,刘永波,高光平..2株金黄色葡萄球菌耐药性分析及中药对其部分耐药基因表达的影响[J].中国畜牧兽医,2023,50(12):5204-5212,9.基金项目
河北科技帅范学院科学研究基金资助(2021YB001) （2021YB001）
河北省现代农业产业技术体系肉牛创新团队(HBCT2023190201) （HBCT2023190201）
河北省重点研发计划项目(22326612D) （22326612D）
河北省奶肉牛呼吸道疾病综合征诊断与防控技术研究(236Z6604G) （236Z6604G）
