中国药房2023，Vol.34Issue(24)：3006-3011,6.DOI:10.6039/j.issn.1001-0408.2023.24.09
蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立
Method establishment of component identification and content determination of 7 components in Mongolian medicine Ixeris chinensis
摘要
Abstract
OBJECTIVE To establish the methods to identify the chemical components of Ixeris chinensis,and determine the contents of 7 components(chlorogenic acid,luteolin,quercetin,rutin,protocatechuic acid,isochlorogenic acid A,luteoloside).METHODS HPLC-Q-Exactive-MS was used to identify the chemical components of I.chinensis.The contents of 7 components in I.chinensis,including chlorogenic acid,were determined by HPLC-MS/MS.RESULTS A total of 45 components were identified in I.chinensis,including 20 organic acids,13 flavonoids,4 fatty acids,4 amino acids,3 nucleosides,and 1 coumarin.The linear range of chlorogenic acid,luteolin,quercetin,rutin,protocatechuic acid,isochlorogenic acid A and luteoloside were 503.00-25 150.00,42.00-2 100.00,5.05-252.50,20.05-1 002.50,25.10-1 255.00,750.00-37 500.00,196.00-9 800.00 ng/mL(r≥0.999 2),respectively.RSDs of precision,stability and reproducibility tests were all less than 3.00%(n=6),and average recovery ranged from 96.72%to 105.84%(all RSD＜4.00%,n=6).The contents of 7 components in 3 batches of I.chinensis were 1 145.77-3 261.25,23.75-97.90,0.92-2.12,1.06-23.18,9.35-21.85,833.25-1 045.58,199.56-1 869.78 μg/g,respectively.CONCLUSIONS The established methods for identification and content determination are rapid and simple,and can be used for the identification of chemical components and the content determination of 7 components in I.chinensis.关键词
山苦荬/高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术/高效液相色谱串联三重四极杆质谱技术/成分鉴别/含量测定Key words
Ixeris chinensis/HPLC-Q-Exactive-MS/HPLC-MS/MS/component identification/content determination分类
医药卫生引用本文复制引用
孙丽君,李君,王秋桐,夏慧敏,王跃武,张谦,张慧文,王焕芸..蒙药材山苦荬的成分鉴别及7种成分含量测定方法建立[J].中国药房,2023,34(24):3006-3011,6.基金项目
内蒙古自治区高等学校科学技术研究项目(No.NJZY22666) （No.NJZY22666）
内蒙古医科大学学科建设项目(No.YDK2022XK011) （No.YDK2022XK011）
内蒙古医科大学蒙药学"一流学科"青年教师科研创新能力提升项目(No.MYX2022-S20) （No.MYX2022-S20）
